To unlock the physiological responses of P. betulaefolia Bunge germplasm resources and identify the elite resources under saline-alkali stress conditions，>3000 seedling plants from 17 betulaefolia Bunge accessions in Gansu，Shanxi and Liaoning provinces were analyzed. The physiological indexes under the mixed stress treatments of neutral salt and alkaline salt were measured，followed by statistical analysis（principal component analysis，heat map，cluster analysis and stress coefficient）. In P. betulaefolia Bunge plants showing strong tolerance，the antioxidant enzyme activity and proline content increased significantly after treatments. Under 300 mmol/L salt-alkali stress，the SOD activity in P. betulaefolia-Hedi 3 and P. betulaefolia-Pinglu 1 was observed with increase up to 75.04% and 70.04%，respectively. The increase on the POD activity in P.betulaefolia-Gansu 1 and P. betulaefolia-Hedi 1 was detected up to 1136.11% and 742.86%，respectively. In P. betulaefolia-Gansu 15 and P. betulaefolia-Gansu 4 a significant increase（344.58% and 343.42%，respectively） on the CAT activity was detected. Moreover，the significant increase on proline content were observed in P. betulaefolia-Pinglu 2，P. betulaefolia-Hedi 1 and P. betulaefolia-Hedi 3 and MDA content were observed in P. betulaefolia-Gansu 16，P. betulaefolia-Gansu 13 and P. betulaefoli-Gansu 1. Except for P. betulaefolia-Gansu 13 that showed salt damage at low concentration（100 mmol/L）under saline-alkali stress，other P. betulaefolia Bunge resources showed degrees of salt damage under 300 mmol/L. By deployment of the principal component analysis，P. betulaefolia-Hedi 1，P.betulaefolia-Hedi 3，P. betulaefolia-Pinglu 1，P. betulaefolia-Gansu 15 and P. betulaefolia-Shanxi 1 showed strong salt-alkali resistance. Based on heat map and cluster analysis，it was found that P. betulaefolia-Gansu 4，P. betulaefolia-Gansu 15，P. betulaefolia-Pinglu 1，P. betulaefolia-Hedi 1 and P. betulaefolia-Hedi 3 had strong salinity tolerance. The coefficient analysis suggested that P. betulaefolia-Gansu 1，P. betulaefolia-Hedi 1，P. betulaefolia-Gansu 16，P. betulaefolia-Gansu 4，P. betulaefolia-Gansu 15 showed strong salt and alkali tolerance. Taken together，P. betulaefolia Bunge. P. betulaefolia-Hedi 1 and P. betulaefoliaGansu 15 are qualified with high saline-alkali resistance，whereas P. betulaefolia-Gansu 13 is saline-alkali sensitive.