湖南省华中樱群体遗传结构和多样性分析
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湖南省植物园

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湖南省林业科技创新杰出青年培养科研项目(XLK202108-6);国家林业和草原局林业科技发展中心项目(KJZXSA2019037);湖 南省林业科技创新专项(XLK201914)


Genetic Structure and Diversity of Cerasus conradinae in Hunan Province
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Hunan Botanical Garden

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Outstanding Youth Training Project for Innovation on Forestry Science and Technology of Hunan Province(XLK202108-6), Forestry Science and Technology Development Project of National Forestry and Grassland Administration(KJZXSA2019037), Special Project for Forestry Science and Technology Innovation of Hunan Province(XLK201914)

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    摘要:

    华中樱是原产于我国的野生樱花,湖南省是其主要分布区域,开展湖南省华中樱遗传多样性研究,可为种质资源保 护及开发利用奠定基础。通过对核糖体基因转录间隔区(ITS,internal transcribed spacer)序列进行 PCR 扩增和测序,应用遗 传学软件分析了湖南省 10 个华中樱群体 207 份个体的遗传多样性和遗传结构。结果表明,湖南省华中樱的总体核苷酸多样 性为 0.0056,单倍型多样性为 0.763,207 份种质资源可分为 14 个单倍型,其中来自怀化市中方县的群体遗传多样性最高,其 次是来自衡阳市南岳区的群体,来自郴州市苏仙区的群体的遗传多样性最低。分子方差分析(AMOVA,analysis of molecular variance)结果表明,60% 的遗传变异存在于华中樱群体间,40% 存在于群体内,大部分群体之间存在较大的遗传分化,其中 来自常德市石门县和来自郴州市苏仙区的群体的遗传分化指数高达 0.96571。中性检验结果表明,Tajima′s D(1.02121)和 Fu and Li′s D(1.07527)均为正值,但统计检验不显著(P>0.10);错配分布分析结果显示,错配分布呈双峰曲线,表明湖南省华中 樱群体遵循中性进化模式,且进化过程中可能经历了平衡选择或突然群体收缩。Mantel test 检验表明,群体之间的地理距离 与遗传距离存在显著的正相关性(r=0.3356,P=0.026<0.05)。湖南省华中樱种质资源遗传多样性水平较高,群体间遗传分化 明显且符合距离隔离模型。

    Abstract:

    To study the genetic diversity of Cerasus conradinae(Koehne)T. T. Yu & C. L. Li so as to contribute to the conservation and utilization of its germplasm,the ITS sequence was amplified by PCR and then sequenced by Sanger method,and genetic analysis software was used to analyze the genetic diversity and genetic structure of 207 individuals from 10 populations of C. conradinae(Koehne)T. T. Yu & C. L. Li in Hunan province. The nucleotide diversity and haplotype diversity of C. conradinae(Koehne)T. T. Yu & C. L. Li in Hunan province was 0.00560,and 0.792 respectively. The 207 germplasms can be divided into 14 haplotypes. Among them,the population from Zhongfang County of Huaihua City has the highest genetic diversity, followed by the population from Nanyue District of Hengyang City,and the population from Suxian District of Chenzhou City had the lowest genetic diversity. The results of AMOVA analysis showed that 60% of the genetic variation existed among the populations of C. conradinae(Koehne)T. T. Yu & C. L. Li,40% existed within the populations,and there was a large genetic differentiation among most of the populations. Among them,the genetic differentiation index between populations from Suxian District and Shimen County was as high as 0.96571. The neutral test results showed that the values of Tajima′s D(1.02121)and Fu and Li′s D(1.07527) are both positive,but statistical tests were not significant(P>0.10),the results of the mismatch distribution analysis showed that the mismatch distribution presented a bimodal curve. This indicated that the population of C. conradinae(Koehne)T. T. Yu & C. L. Li in Hunan province followed a neutral evolutionary model,and might have experienced balanced selection or sudden population shrinkage during the evolution process. Mantel test showed that there was a positive correlation between geographic distance and genetic distance among the populations(r=0.3356,P=0.026<0.05). The genetic diversity of C. conradinae(Koehne)T. T. Yu & C. L. Li germplasms in Hunan province was relatively high,and the genetic differentiations among populations were obvious and conformed to the Isolation-by-distance(IBD)model.

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严佳文,柏文富,李建挥,等.湖南省华中樱群体遗传结构和多样性分析[J].植物遗传资源学报,2022,23(2):376-384.

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  • 收稿日期:2021-08-12
  • 最后修改日期:2021-08-25
  • 录用日期:2021-09-14
  • 在线发布日期: 2022-03-10
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