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抗胞囊线虫2号生理小种大豆种质的评价和利用
魏 荷, 练 云, 李金英, 李海朝, 周 扬, 雷晨芳, 张 辉, 武永康, 王仕伟, 王金社, 卢为国
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(河南省作物分子育种研究院/国家大豆改良中心郑州分中心/农业农村部黄淮海油料作物重点实验室/河南省油料作物遗传改良重点实验室)
摘要:
大豆胞囊线虫(Soybean cyst nematode,SCN)2号生理小种是黄淮海地区的优势小种。自2017年起对333份大豆种质进行2号生理小种的抗性鉴定,筛选出抗性资源19份,其中表现抗病5份、中抗14份。通过KASP标记对调控大豆抗胞囊线虫病主效位点rhg1和Rhg4检测,19份抗性种质均含有抗性位点;仅圆黑豆(ZDD02481)的主效位点rhg1和Rhg4连锁的KASP标记的基因型和抗病对照品种PI437654一致,其余均和抗病对照品种PI90763一致。以其中1份抗性材料灰皮支黑豆(ZDD02315)为父本,创制出的2份抗性种质郑12915和郑12929,均含有抗性位点rhg1和Rhg4,农艺性状优于灰皮支黑豆。本研究结果为大豆抗胞囊线虫育种提供依据和材料。
关键词:  大豆种质  胞囊线虫  2号生理小种  抗性评价  KASP标记
DOI:10.13430/j.cnki.jpgr.20210721003
投稿时间:2021-07-21修订日期:2021-10-10
基金项目:国家重点研发计划课题(2016YFD0100201);国家自然科学基金(31901501,31371652);河南省农业科学院科研项目(大豆种质资源收集、整理与保存)
Evaluation and Utilization of Soybean Germplasm Resistant to Race 2 of Soybean Cyst Nematode
WEI He, LIAN Yun, LI Jin-ying, LI Hai-chao, ZHOU Yang, LEI Chen-fang, ZHANG Hui, WU Yong-kang, WANG Shi-wei, WANG Jin-she, LU Wei-guo
(Henan Academy of Crop Molecular Breeding, Henan Academy of Agricultural Sciences / Zhengzhou Subcenter of National Soybean Improvement Center/Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crops Improvement)
Abstract:
Soybean cyst nematode (SCN) race 2 is predominant in the Huang-huai Rivers valley. Out of 333 soybean accessions that have been tested for the resistance to SCN race 2 (SCN 2) since 2017,19 resistant accessions including 5 resistant and 14 moderately resistant have been identified. Genotyping using KASP markers showed that all the 19 resistant accessions harbored two resistant loci rhg1 and Rhg4. The genotypes of KASP markers linked to rhg1 and Rhg4 for 18 accessions were the same as PI90763,while Yuanheidou (ZDD02481) was consistent with PI437654. Moreover,resistant genotype Zheng 12915 and Zheng 12929,which were developed from resistant germplasm Huipizhiheidou (ZDD2315) showed agronomic performance better than the parental line. Collectively,these results provided a basis for SCN breeding in soybean.
Key words:  Soybean germplasm  Cyst nematode  Race 2  Evaluation of resistance  KASP marker

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