薏苡属种质资源Waxy基因分型及优异资源筛选
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作者单位:

1.贵州黔西南喀斯特区域发展研究院;2.中国农业科学院作物科学研究所;3.贵州省薏苡工程技术研究中心

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基金项目:

贵州省现代农业产业体系(黔财农[2019]15号);贵州省农业技术服务资金(黔财农[2020]51号);贵州省科技计划项目(黔科合支撑[2019]2306号);黔西南州科技计划项目(2020-1-03)


The Waxy Trait Genotyping and Elite Lines Selection in Adlay (Coix L.) Germplasms
Author:
Affiliation:

1.Southwest Guizhou Institue of Karst Regional Development;2.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences;3.Adlay of Engineering Technical Research Centre in Guizhou

Fund Project:

Construction of Modern Agricultural Industrial System in Guizhou Province(No.201915),Guizhou Agricultural Service Fund(No.202051),Science and Technology Program in Guizhou Province(No.20192306),Southwest Guizhou of Science and Technology Program (No.2020-1-03)

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    摘要:

    薏苡是重要的药食同源作物,在膳食结构多样性生产中具有重要意义。本研究旨在深入分析薏苡种质资源的粳、糯性特征,并筛选优异资源作为品种选育和生产应用的基础材料。通过大田试验进行表型分析,供试资源的农艺性状和籽粒性状均表现为丰富的遗传多样性。利用碘试剂快速染色法对116份薏苡属资源进行胚乳类型鉴定,并通过Waxy基因分型技术筛选优异薏苡资源。结果显示,在全部资源中,29份野生型资源糯性籽粒百分率在0%~25.00%之间,均表现出粳性(非糯性)特征。87份栽培资源中粳、糯性胚乳类型均存在,包括纯粳性资源8份,纯糯性资源17份,62份材料以两种胚乳类型的混杂形式。根据表型鉴定和单籽粒的粳糯性分型,筛选出16份遗传稳定、性状差异明显的优良株系,其中,糯性材料14份,其总淀粉含量为57.82%~71.51%,直链淀粉含量为0%~4.98%;粳性材料2份,总淀粉含量分别为65.73%和65.65%,直链淀粉含量分别为23.75%和25.48%。PCR检测结果显示,14份糯性品系扩增出单一的550 bp片段,2份粳性品系扩增出单一的约850 bp片段。糯性和粳性株系材料的直连淀粉和支链淀粉含量差异明显,而且Waxy位点基因型的纯合、遗传稳定,可作为薏苡品质改良和品种选育的基础遗传材料。

    Abstract:

    Adlay (or Job’s tears) is a cereal crop used for food and Chinese herb, and it is a necessary supplement in dietary structure diversity. In order to select germplasm resources in breeding procedure and production application, this study analyzed the endosperm type in adlay. It was shown by field tests that all germplasm accessions represented rich genetic diversity in the main agronomical traits. Endosperm type was identified and elite lines were selected from 116 adlay germplasms by rapid iodine reagent dyeing and Waxy genotyping methods. Twenty-nine wild germplasms were nonglutinous (none-waxy), and the waxy grain ratio was 0~25.00%. However, both types, either waxy or none-waxy, were found in 87 cultivated accessions including 8 nonglutinous varieties and 17 waxy varieties. Two types were simultaneously detected in 62 cultivar germplasms. Sixteen elite lines including 14 waxy and 2 nonglutinous were identified based on the agronomical performance and endosperm types. In waxy lines total starch and amylose contents were 57.82%~71.51% and 0~4.98%, respectively, while in two nonglutinous lines the total starch content was 65.73% and 65.65%, and amylose content was 23.75% and 25.48%. PCR amplification in waxy lines produced a 550 bp fragment, while in nonglutinous lines a ca. 850-bp fragment was detected. The contents of amylose and amylopection were differed obviously, and Waxy genotype were homozygous and stable in heredity, which could be used as fundamental genetic stocks in quality modification and genetic improvement in adlay.

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李祥栋,潘虹,魏心元,等.薏苡属种质资源Waxy基因分型及优异资源筛选[J].植物遗传资源学报,2022,23(1):183-194.

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  • 收稿日期:2021-07-08
  • 最后修改日期:2021-07-18
  • 录用日期:2021-08-03
  • 在线发布日期: 2022-01-07
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