FPF1 (flowering-promoting factor 1) is one of the important family involved in the genetic control of flowering time in plant. So far, the knowledge concerning the FPF1 family in Aechmea fasciata has been very much limited. In this study, the AfFPF1 gene was cloned from A. fasciata, a tropical flower, based on the transcriptome sequencing data. The protein encoded by this gene contained 103 amino acids and had a molecular mass of 12.06 kD. The AfFPF1 gene was ectopically expressed in Arabidopsis thaliana, which advanced the flowering time of A. thaliana, reduced the number of rosette leaves and promoted the growth of root system. Some flowering-promoting genes such as AtFT, AtAP1, AtLFY and AtSOC1 were found to be significantly increased in the transgenic plants, while the expression of the flowering-inhibiting gene AtFLC was down-regulated. This further confirmed that AfFPF1 could positively regulate the flowering time in A. thaliana and might interact with these genes. These results demonstrated that AfFPF1 might be involved in regulating the flowering process of Aechmea fasciata, providing a theoretical basis of characterization of AfFPF1, the regulation of A. fasciata flowering using genetic approach and the molecular mechanism of ethylene-induced flowering in the near future.