One inbred of maize Reid-Lan., and 6 inbreds of maize RLT (Reid-Lan. × Tuxpe?o) were selected for testing, with 5 representative inbreds derived from 5 different subgroups as testers. 35 hybrids obtained from crossing the 12 inbreds by using the NCII genetic mating design and grown in Guiyang and Dejiang of Guizhou Province were evaluated for their yield-related traits, and the 12 inbreds were evaluated by using the gene chip MaizeSNP50 containing 60,000 SNPs, in order to enhance the utilization potential of Tuxpe?o and to take advantage of the genetic prepotency of Reid-Lan. germplasm in breeding. The results showed that the inbred QB2539 derived from RLT had the highest GCA of 7.28, which was higher than that of QB662 derived from Reid-Lan. The genetic distance (GD) value between Reid-Lan. germplasm inbred QB662 and QR273 was 0.3689, and the GD value between Tuxpe?o germplasm inbred S122 and QR273 was 0.3554, whereas the GD value between RLT germplasm inbred QB2539 and QR273 was 0.3732. Hybrids from the combination of RLT and Suwan germplasm were the top three among all the 35 combinations in regard to yield, among which QB2539 × QR273 had the highest yield, with a 29.66% increase over the CK of Qiandan 16. These results revealed the potential of RLT germplasm for wide use in maize breeding, and would provide some new improved methods and material support for maize germplasm innovation.