Using the NPR1 gene family member’s protein sequences from Arabidopsis as query, MaNPR1 gene family members were identified in the banana A genome database, and these features using bioinformatics methods and the expression upon infection with Foc TR4 were further investigated. In total, 15 family members were identified, designated MaNPR1-MaNPR15. Physicochemical properties, conserved functional domain, essential amino acid residues and motif analysis results of MaNPR1 gene family are highly consistent with those of other species. The classification of phylogenetic tree, gene structure and functional domain suggested a functional specificity among members of the MaNPR1 gene family. The interspecific phylogenetic tree showed that the MaNPR1 gene family were divided into three subgroups, co-residing with respective Arabidopsis NPR1 members in each group. The predominant-cultivating banana variety ' Cavendish ' (Musa acuminata L.AAA group) was highly susceptible to fusarium wilt caused by Fusarium oxysporum f. sp. Cubense Tropical Race 4（Foc TR4）. By analyzing the transcriptome dataset from root transcriptome data of infected and un-infected Brazilian samples, eight differentially expressed MaNPR1 genes were identified, which were further tested by RT-qPCR in ‘Cavendish’ and the resistant variety ‘GCTCV-119’. Two members MaNPR4 and MaNPR11 showed an increasing transcriptional levels at time-points in ‘GCTCV-119’, in relative to the constant reduction in ‘Cavendish’. That indicated an involvement of MaNPR4 and MaNPR11 in responses to fusarium wilt. Taken together, this study raised a potential in breeding for fusarium wilt resistance banana by use of NPR1 genes.