马铃薯抗褐变种质资源的鉴定与筛选
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黑龙江省农业科学院克山分院;农业部马铃薯生物学与遗传育种重点研究室,黑龙江省农业科学院克山分院;农业部马铃薯生物学与遗传育种重点研究室,黑龙江省农业科学院克山分院;农业部马铃薯生物学与遗传育种重点研究室,黑龙江省农业科学院克山分院;农业部马铃薯生物学与遗传育种重点研究室,黑龙江省农业科学院克山分院;农业部马铃薯生物学与遗传育种重点研究室,黑龙江省农业科学院克山分院;农业部马铃薯生物学与遗传育种重点研究室,黑龙江省农业科学院克山分院;农业部马铃薯生物学与遗传育种重点研究室

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黑龙江省农业科技创新工程(2014QN003);农业部“948”项目(2015-Z52);国家科技支撑计划 (2012BAD02B05);黑龙江省应用技术研究与开发(GA15B102-03);齐齐哈尔市农业公关项目(NYGG-201504)


Identification and Selection forPotato Anti-browning Germplasm Resources
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    摘要:

    筛选抗褐变马铃薯基因,创造抗褐变新种质,培育抗褐变马铃薯新品种,旨在通过育种技术手段解决马铃薯加工中褐变现象。选用目前育种资源材料275份,通过测定褐化指数、褐化强度、煮后变褐三个指标来筛选抗褐变材料。结果表明:(1)对褐化指数进行聚类分析,第I类为高抗褐变材料,褐化指数0.00-16.67,13份材料;第II类为抗褐变材料,褐化指数25.00-54.17,34份材料;第III类为耐褐变材料,褐化指数62.50-83.33,50份材料;第IV类为严重褐变材料,褐化指数87.50-95.83,38份材料;第V类为完全褐变材料,褐化指数为100,140份材料。(2)将30 ℃褐化20 min时褐化强度小于0.25,4 ℃放置24 h后,变化值不超过0.15的材料筛选出来,作为抗褐变材料。通过褐化强度共筛选出材料25份。(3)马铃薯煮后变褐高值为9,低值为5,大部分品种均可用于鲜食消费。三指标最终筛选出高抗褐变材料4份,云薯501、CIP395109.29、CIP393615.6、云薯401;抗褐变材料4份,讷河高淀粉、CIP397100.9、克200950-3、s.goniocalyx;耐褐变材料4份,克新22号、CIP393617.1、Andover、克9412-13。这些材料可作为抗褐变马铃薯新品种选育的基础材料。

    Abstract:

    The objective of this study was to select anti-browning potato gene and create new anti-browning potato germplasm resources, further to breed new anti-browning potato varieties. We hoped to use breeding technology to solve the browning phenomenon in potato processing.275 breeding varieties were selected as test materials. To select anti-browning varieties, we mensurated the three indexes, browning index, browning degree and ACD. (1)The cluster analysis of browning index showed that the varieties can be divided into 5 types. The first type was higher anti-browning material, browning index 0.00-16.67, 13 materials; the second type was high anti-browning material, browning index 25.00-54.17, 34 materials; the third type was anti-browning material, browning index 62.50-83.33, 50 materials; the fourth type was browning material, browning index 87.50-95.83, 38 materials; the fifth type was the complete browning material, browning index was 100,140 materials. (2)Browning degree was less than 0.25 when browning time was 20 min for 30℃, and after 4℃for 24h the variation value was no more than 0.15,which can be selected as anti-browning resources. A total of 25 materials were selected by browning degree. (3) Most potato resources can be used for fresh food consumption. The maximum value of ACD was 9, and the minimum was 5. There were 4 higher anti-browning materials, Yunshu 501,CIP395109.29, CIP393615.6,Yunshu 401; 4 high anti-browning materials, Nehegaodianfen, CIP397100.9,Ke200950-3, s.goniocalyx; 4 anti-browning materials,Kexin22, CIP393617.1,Andover,Ke9412-13. These 12 selected materials can be used as the basic material for breeding new potato anti-browning varieties.

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王海艳,王立春,李凤云,等.马铃薯抗褐变种质资源的鉴定与筛选[J].植物遗传资源学报,2018,19(2):263-270.

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  • 收稿日期:2017-07-27
  • 最后修改日期:2017-08-31
  • 录用日期:2017-09-28
  • 在线发布日期: 2018-03-19
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