The hybridization composition, which parents was high oleic acid line G63 with pink seed coat and normal oleic acid line VG-01 with purple and white variegated seed coat, was used in this study. 16 true F1 hybrids were identified by allele-specific PCR (AS-PCR) methods. The genotype _ol1_ol2 individuals were screened in F2 population and the seed coat color segregation in 64 F2:3 family lines was checked by Chi-square test. The results showed that the full-color peanut: variegated color peanut was 9:7 at 0.05 level. The variegated seed coat was controlled by two pairs of complementary genes. When the two pairs of genes were dominant, the seed coat showed full color. At least one pair of genes for recessive homologous, the seed coat showed variegated color. The genotypes of 334 F3 plants with variegated seed coat were identified by AS-PCR, and 29 plants of ol1ol1ol2ol2 genotypes were selected. The results of gas chromatography showed that GC value of F4 seed ranged from 70.77% to 82.87%, and oleic acid / linoleic (O/L) ranged from 8.15 to 26.30. The main stem of F4 population was 12.4 cm - 87.3 cm, with an average of 57.2 cm, which was 34.97% higher than that of the control Jihua 2. The first pair of lateral branches was 91.6 cm - 196.3 cm, with an average of 134.1 cm, which was 34.28% higher than that of the control. Pod weight per plant, pod number per plant, kernel weight per plant and kernel number per plant were (27.16 ± 9.51) g, (22.11 ±10.26), (20.10 ± 7.17) g and (31.94 ±15.16) respectively. The new germplasm lines (9-7, 14-2, 14-3, 17-3 and 17-7) were all red and white variegated seed coat. Comparied with the control, pod weight per plant was increased 230.02%, 165.80%, 210.66%, 200.65% and 160.26% respectively and pod number per plant was increased 280.00%、340.00%、450.00%、210.00% and 150.00% respectively. The oleic acid GC value was 80.54%, 81.75%, 80.63%, 81.30% and 81.56% respectively. The new germplasm materials not only have high oleic acid content, but also have great health care value, which have great practical significance to enrich the genetic diversity of high oleic peanut in China.