谷子窄颖花突变体sins1的表型分析和突变基因定位
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国家现代农业产业技术体系(CARS07-13.5-A02);中国农业科学院创新工程杂粮团队;国家863计划(2013AA102603)和国家自然科学基金(31301328, 31522040)项目资助


Morphological characterization and Gene Mapping of sins1 (narrow spikelet 1) in Foxtail Millet
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    摘要:

    利用甲基磺酸乙酯(Ethyl methylsulfonate,EMS)诱变剂处理野生型Yugu1(豫谷1号),在后代中发现了一个可以稳定遗传的颖花明显变窄的突变体,将其命名为sins1。该突变体同时伴随有株高降低、穗部变短变细、旗叶变短变窄、单穗谷码数减少,千粒重降低等变异,且均达到显著水平(P≤0.05)。与Yugu1相比,突变体sins1的株高显著降低了3.89 %,穗长和穗粗分别显著降低了17.41 %和21.62 %,旗叶叶长和叶宽分别显著降低了15.07 %和25.87 %,千粒重显著降低了41.09 %, 谷码数显著降低了25 %。利用突变体 sins1为母本、SSR41为父本构建F2定位群体,F2代正常颖花与窄颖花植株数目的分离比例为3:1,表明该突变性状由隐性单基因控制。利用F2 代群体隐性单株,最终将突变基因定位在3号染色体上3-2658 与CAAS3031 间约7.709 Mb的距离内,为下一步精细定位提供了基础,同时也为促进禾本科作物颖花的研究提供了方向。

    Abstract:

    A foxtail millet narrow spikelet mutant which was induced from Yugu1 by EMS treatment, was genetically identified and named as sins1. The mutant exhibited narrow-spikelet, low-plant-height, short-thin-panicle, short-narrow-leaf, low-spikelet-number per panicle, as well as low-1000-grain weight. Compared with its wild-type Yugu1, plant height, panicle length, panicle diameter, flag-leaf length, flag-leaf width, the 1000-grain weight, spikelet number per panicle of sins1 were decreased by 3.89 %, 17.41 %, 21.62 %, 15.07 %, 25.87 %, 41.09 %, 25 % respectively (P≤0.05). Genetic analysis suggested that its F1 hybrid plants were restored to wild type and 3:1 segregation ratio of wild type to mutant plants were observed in the F2 generation of sins1×Yugu1, suggesting that the narrow-spikelet trait of sins1 mutant is controlled by a single recessive nuclear gene. Genetic mapping of the mutant gene was conducted using narrow-spikelet individuals from the F2 segregating population of sins1×SSR41. Finally, the mutant gene was mapped into genomic region within SSR markers 3-2658 and CAAS3031 in chromosome 3, with a 7.709 Mb interval. This research had laid the foundation for fine-mapping of the narrow spikelet causal gene and will promote spikelet development related research in cereal crops.

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张 浩,汤 沙,李迎涛,等.谷子窄颖花突变体sins1的表型分析和突变基因定位[J].植物遗传资源学报,2017,18(3):538-545.

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  • 收稿日期:2016-11-14
  • 最后修改日期:2017-02-27
  • 录用日期:2017-03-10
  • 在线发布日期: 2017-05-19
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