绿豆种质资源的ISSR遗传多样性分析
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?国家现代农业产业技术体系“食用豆体系栽培与土肥岗位”(nycytx-18-G12);


Analysis on Genetic Diversity of Vigna radiate Germplasm Resources by ISSR
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    摘要:

    利用ISSR标记对33份绿豆种质进行了遗传差异和亲缘关系分析。 结果表明,从46条ISSR引物中筛选出10条扩增条带清晰、多态性高的引物,利用这10条引物从33份绿豆种质中共扩增出118条条带,其中多态性条带115条,多态性位点比例98.18%。遗传相似性与聚类分析结果表明,供试绿豆种质间的遗传相似系数范围为0.50~0.98之间,平均0.68。当遗传相似性系数为0.682时,供试33份绿豆种质资源分为4个ISSR类群( ISSR Groups,IGs),第Ⅰ类群包括产地为黑龙江、吉林共9份和河北的1份绿豆种质资源,第二类群包括河南、山西和陕西的所有和河北的4份绿豆种质资源,第三类群为来自泰国的5份绿豆抗虫资源,第四类群包括山东和内蒙各2份绿豆资源;当遗传相似系数为0.98时,供试的33份绿豆种质资源可被全部区分开。 ISSR分析结果表明, ISSR类群划分与绿豆的地理来源存在一定相关性;而同一类群中,地理来源相同的绿豆种质间也存在一定的遗传差异。

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    To clarify genetic difference and relationship among Vigna radiate cultivars from different regions, ISSR molecular marker technique was used. It is showed that 10 primers with clear amplified bands and high polymorphism which selected from 46 primers were amplified a total of 118 fragments from 33 cultivars of Vigna radiate germplasm resources ,in which there were 115 polymorphic fragments and the ratio of polymorphic sites was 98.18%. The genetic similarity and cluster analysis indicated that the genetic similarity coefficient varied from 0.50 to 0.98 and the average coefficient was 0.68. All of the tested Vigna radiate were divided into four ISSR groups when the genetic similarity coefficient was 0.682. Nine cultivars came from Heilongjiang and Jili and one cultivar came from Hebei were grouped into IG-I; 14 cultivars were grouped into IG-Ⅱ, with four cultivars from Hebei, and the rest from Henan, Shanxi and Shanxi; five cultivars of insect-resistant Vigna radiate came from Thailand were grouped in IG-Ⅲ; four cultivars from Shandong and Inner Mongolia were grouped into IG-Ⅳ,with two cultivars from each province. All the tested Vigna radiate can be distinguished when the genetic similarity coefficient was 0.98. The ISSR genetic diversity analysis showed that there may be some correlation between the ISSR grouping and the geographic origin of Vigna radiate, while there is genetic diversity in the same germplasm resources which come from the same geographic origin.

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赵雪英,王宏民,李赫,等.绿豆种质资源的ISSR遗传多样性分析[J].植物遗传资源学报,2015,16(6):1277-1282.

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  • 收稿日期:2015-01-12
  • 最后修改日期:2015-04-09
  • 录用日期:2015-08-06
  • 在线发布日期: 2015-11-04
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