PEG-6000胁迫下裸燕麦萌发期抗旱性鉴定与评价
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国际生物多样性中心合作项目(CCAFS):APO12/003;农业部作物种质资源保护专项(NB2012-2130135-25-06)


Identification and Evaluation of Drought Resistance of Naked Oat (Avena nuda L.) under PEG-6000 stress at Germination Stage
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    摘要:

    以17个裸燕麦品种为试材,测定了20% PEG-6000水溶液模拟干旱胁迫下各品种的发芽势、发芽率、发芽指数等11个鉴定指标。以鉴定指标的相对值为抗旱性评价指标,在主成分分析的基础上,利用加权隶属函数法对供试裸燕麦品种的抗旱性进行了综合评价,然后对不同鉴定指标在抗旱性评价中的作用进行了比较分析。结果表明:不同裸燕麦品种的抗旱性可由不同鉴定指标来体现;鉴定指标中的种子活力指数(VI)受干旱胁迫的影响最大,并与萌发期抗旱性的关系最为密切,可用于裸燕麦种质萌发期抗旱性的快速或初步鉴定;通过测定发芽率、平均发芽速度、最长初生根长、初生根总长、胚芽鞘长、种子活力指数,可有效预测裸燕麦品种萌发期的抗旱性;通过比较供试品种的抗旱性综合评价值(D值),筛选出N-C33Ⅳ-45-16、grosse和高千四号三个抗旱性较强的裸燕麦品种,可用于抗旱栽培或抗旱育种。

    Abstract:

    Germination energy, germination rate, germination index and other eight identification indices of seventeen varieties of naked oat were measured under drought stress simulated with 20% PEG-6000. According to the relative value of eleven identification indices as evaluation indices, drought resistance of oat varieties was evaluated comprehensively with the method of weighted membership function on the basis of the principal component analysis. The role that identification indices play in evaluation of drought resistance among different varieties was compared. The results showed that vigor index (VI) was the most susceptible to drought stress, and the closest to the comprehensive evaluation value of drought resistance and could be used for rapid and preliminary identification of drought resistance of naked oat germplasm at germination stage. An optimal regression model for the comprehensive evaluation of drought resistance was established and could be use to effectively predict the drought resistance of naked oat varieties at germination period through measuring germination rate, average germination velocity, length of the longest primary root, total length of primary roots, coleoptile length and vigor index of seed. Three varieties, namely N-C33Ⅳ-45-16, grosse and Gaoqian Sihao with stronger resistance to drought stress were identified through comparing the comprehensive value of drought resistance among tested varieties, and could be used to cultivation and breeding for dry conditions.

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陈新,宋高原,张宗文,等. PEG-6000胁迫下裸燕麦萌发期抗旱性鉴定与评价[J].植物遗传资源学报,2014,15(6):1188-1195.

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  • 收稿日期:2014-02-11
  • 最后修改日期:2014-03-12
  • 录用日期:2014-10-08
  • 在线发布日期: 2014-10-08
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