卵圆型软条白沙枇杷变异种质鉴定研究
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Identification of Egg-shaped Ruantiao Baisha Loquat Mutant Germplasm
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    本文以卵圆型软条白沙枇杷变异种质为试材,母株软条白沙及中国、日本、新西兰3个国家的30个白沙枇杷栽培品种为对照,以形态学性状调查为基础,结合SRAP分子标记对变异种质进行鉴别研究。SRAP标记从96对引物中筛选出18对进行扩增,结果共获得135个位点,其中102个多态性位点,多态性百分率为75.6%。不同品种间的遗传相似系数(Genetic Similarity,GS)在0.53~0.89之间。在GS为 0.65处,可将31份枇杷种质分为四类。Ⅰ:均为来自中国浙江的8份种质。 Ⅱ:为 来自日本、新西兰和中国江苏和福建的19个种质,GS在0.58~0.89之间。Ⅲ:为上海白沙和广西白蜜枇杷两个种质,GS为0.69。Ⅳ:为福建白梨和龙才白两个种质,GS为0.69 。作为重点研究对象,卵圆形软条白沙与对照相似系数处于0.55~0.88之间;与白荔枝的遗传相似度最大(GS=0.88);与乌躬白遗传相似度最小(GS=0.55);与母株软条白沙的相似系数为仅0.73,两者的遗传相似度不大。生物学性状调查表明,卵圆形软条白沙变异材料与母株软条白沙在果形、种子数和可食率等性状上具有明显差异。综合研究结果,可见卵圆形软条白沙与母株软条白沙相比,综合性状发生了明显的遗传变异,它应属芽变种质(材料)。本研究对枇杷变异材料的创新利用、新品种审定与知识产权保护具有重要的实际意义。

    Abstract:

    The loquat germplasm of egg-shaped Ruantiao Baisha mutant, Ruantiao Baisha and five domestic cultivars were used to identify the variation of germplasm using morphological traits investigation and SRAP. 18primers which could produce clear and polymorphic bands were screened out from 96 ISSR random primers. 135 locis were detected ,of which 102were polymorphic and the polymorphic percentage was 75.6%.The genetic similarity coefficients of the loquat germplasms were among 0.53~0.89. 31 white flesh loquat germplasms were divided into four groups when the similar coefficient was 0.65. All the loquat germplasms come from Zhe Jiang province were clustered together in the first group.The second group contain 19 germplasms coming from China ,Japan and New zealand. .The genetic similarity coefficients between 0.58 and 0.89. The third group contain Shanghai Baisha and Guangxin Bami. The fourth group include Baili and Longcai Bai .As the key research object, GS between egg-shaped Ruantiao Baisha and reference were among 0.55~0.88. It had the maximum genetic simlity with Bai Lizhi, and minimun genetic simlity with Wu Gong Bai. GS of egg-shaped Ruantiao Baisha and Ruantiao Baisha was only 0.73. The study of biological characters, There were obvious differences among fruit shape,seed number and edible rate of egg-shaped Ruantiao Baisha and Ruantiao Baisha The results showed that the egg-shaped Ruantiao Baisha mutant and Ruantiao Baisha loquat has obvious difference in characters such as fruit shape, seed numbers and edible rate. The analysis confirmed the obvious genetic variation between the egg-shaped Ruantiao Baisha and Ruantiao Baisha loquat. This study is important to apply new varieties identity and protection of intellectual property rights for the egg-shaped Ruantiao Baisha mutant.

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陈方永,谢丽雪,王引,等.卵圆型软条白沙枇杷变异种质鉴定研究[J].植物遗传资源学报,2014,15(5):986-991.

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  • 收稿日期:2014-01-29
  • 最后修改日期:2014-03-05
  • 录用日期:2014-08-07
  • 在线发布日期: 2014-09-10
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