紫花苜蓿NAC转录因子MsNAC1基因的克隆、生物信息学分析及非生物逆境胁迫下的表达分析
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然基金(31360572);内蒙古自然科学基金项目(2011MS0408);内蒙古高等学校科学研究项目(NJZY11219)


Cloning and Bioinformatics Analysis of an Novel NAC Transcription Factor MsNAC1 from Medicago sativa L. and Detection of its Expression Under Abiotic Stresses
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    NAC转录因子是高等植物所特有的具有多种生物功能的转录调控因子,在植物生长发育、抵抗逆境和激素调节等方面发挥着重要作用。本研究利用RACE-PCR技术,克隆了紫花苜蓿NAC转录因子MsNAC1基因的cDNA序列。生物信息学分析显示MsNAC1基因的开放阅读框(ORF)为993bp,编码了一个由330个氨基酸残基组成的稳定的亲水性蛋白MsNAC1,N-端具有保守的NAM结构域,C-端高度变异,具备了NAC转录因子的基本特征。MsNAC1蛋白被定位在细胞核中,含有2条核定位信号序列,具有9个糖基化位点和23个磷酸化位点,三级结构为对称的同型二聚体。多重比对发现MsNAC1蛋白与豆科植物NAC类蛋白的同源性较高,与禾本科的较低;系统进化分析发现MsNAC1蛋白属于NAC转录因子家族中的NAM亚族,与GmNAC048的亲缘关系最近。利用qRT-PCR分析MsNAC1基因在非生物逆境胁迫下的表达情况发现,在高盐、干旱和低温胁迫下均有不同程度的上调,说明该基因可能参与调控非生物逆境胁迫的生理响应;并且根中的表达量上调幅度总体高于叶片,且低温胁迫下根和叶中的表达量均最高、持续时间最长,说明该基因可能与根的耐低温性有较高的相关性。

    Abstract:

    NAC are specific novel transcription factors in plants with multiple biological functions, which play an important role in growth and development, anti-stress and hormonal regulation. In this study, the MsNAC1 gene in Medicago sativa L. was obtained by rapid amplification of cDNA ends (RACE) method. Bioinformatics analysis showed that MsNAC1 has an open reading frame (ORF) of 993 bp, encoding a stable, water-soluble protein with 330 amino acids. The MsNAC1 protein with a conserved NAM domain in the N-terminus and highly divergent C-terminus had the basic characteristics of the NAC transcription factors. It was predicted to locate in the nucleus with 2 nuclear localization sequences, and contain 9 glycosylation sites and 23 phosphorylation sites. The tertiary structure of MsNAC1 was predicted to be dimer. Sequence alignment analysis revealed that MsNAC1 has high homology with legume plants NAC protein but low with Gramineae. Phylogenetic analysis revealed that MsNAC1 was a member of NAC transcription factor family belonging to NAM subgroup, and has most close genetic relationship with GmNAC048. The expression of MsNAC1 under abiotic stresses was analyzed by qRT-PCR, its expression levels were upregulated in high-salinity, drought and cold stress treatments, indicating that this gene may be participate in regulation of plant abiotic stress response. The expression level in root was higher than that in leaf, and was highest and longest in root and leaf in cold stress treatment, indicating that this gene may be highly related to low temperature resistance in root.

    参考文献
    相似文献
    引证文献
引用本文

申玉华,徐振军,杨晓坡,等.紫花苜蓿NAC转录因子MsNAC1基因的克隆、生物信息学分析及非生物逆境胁迫下的表达分析[J].植物遗传资源学报,2014,15(6):1312-1319.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2014-01-20
  • 最后修改日期:2014-03-04
  • 录用日期:2014-10-08
  • 在线发布日期: 2014-10-08
  • 出版日期:
您是第位访问者
ICP:京ICP备09069690号-23
京ICP备09069690号-23
植物遗传资源学报 ® 2024 版权所有
技术支持:北京勤云科技发展有限公司