Resistance evaluation and gene postulation of the eight wheat breeding parents were carried out by using 19 Puccinia triticina races with different virulent patterns at seedling stage and adult stage. 31 molecular markers closely linked or co-segregated with 24 wheat leaf rust resistance genes were used to test the 8 wheat breeding parents. The results indicated that lines L83#-5 and L83#-6 carried Lr1, might contain Lr2c or Lr42. L/PL2003-1 carried Lr1, might contain Lr2c, Lr28 or Lr42. Guinong 13 might contain Lr28. Line 92R137 might contain Lr2c or Lr28. Line L201 carried Lr1, might contain Lr2c, Lr16 or Lr28. TM carried Lr41 and unknown resistance genes. The results also indicated that the resistance of TM is the strongest in the 8 wheat accessions, and will be a good accession in wheat leaf rust resistance breeding programs.