河北省花生地方品种基于EST-SSR的遗传多样性及性状-标记相关分析
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Genetic diversity and marker-trait analysis for the peanut landraces in Hebei province based on EST-SSR markers
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    摘要:

    利用215对EST-SSR引物对70份河北省花生地方品种的遗传多样性进行扫描,结果表明,58对引物在不同材料间具有多态性,多态性引物比率为26.98%。共扩增出168个位点,其中156个为多态性位点;不同EST-SSR的多态性信息指数PIC变幅为0.063-0.9825,平均为0.5244。聚类分析结果表明,在阈值为8.00时,可以将各地方品种明显分为两大类,第Ⅰ类群为普通型,第Ⅱ类群为珍珠豆型和多粒型,与植物学分类一致。单标记-性状相关分析表明,同一标记位点可与多个性状相关,获得与5个农艺性状显著相关的标记12对。

    Abstract:

    A total of 215 pairs of EST-SSR primers were used to screen the genetic diversity of peanut landraces in Hebei Province. The results showed that 58 pairs of EST-SSR primers were polymorphism with a polymorphic rate of 26.98%. One hundred and sixty eight alleles were detected in different materials and 156 of which were polymorphic loci. The polymorphic information content (PIC) of each primer varied from 0.063-0.9825 with an average of 0.5244. Cluster analysis was conducted and showed that materials tested could be divided into 2 groups, the first group was A. hypogaea and the second group was A. vulgaris and A. fastigiata, which was consistent with the botanical classification. The trait and marker correlation analysis revealed that one marker could be related with different kinds of traits and 12 pairs of EST-SSR markers were selected with significant correlation with 5 different kinds of agronomic traits.

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胡宏霞,穆国俊,侯名语,等.河北省花生地方品种基于EST-SSR的遗传多样性及性状-标记相关分析[J].植物遗传资源学报,2013,14(6):1118-1123.

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历史
  • 收稿日期:2013-03-10
  • 最后修改日期:2013-04-23
  • 录用日期:2013-06-13
  • 在线发布日期: 2013-09-24
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