纤毛鹅观草DREB类基因RcDREB1的克隆及其蛋白结合干旱应答元件DRE的功能验证
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转基因生物新品种培育重大科研专项基金;中国博士后科学基金


Cloning of a DREB-like Gene RcDREB1 in Roegneria ciliar and Functional Identification of Its Protein Binding Specificity to the Dehydration-responsive Element (DRE)
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Genetically modified organisms to cultivate new varieties of major scientific research funds ; China Postdoctoral Science Foundation

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    摘要:

    以纤毛鹅观草为材料,通过RT-PCR和RACE技术,作者首次克隆了一个纤毛鹅观草DREB类基因的全长cDNA序列,命名为RcDREB1。该基因编码蛋白含有一个保守的AP2结构域,表明该基因是AP2大家族的一个新成员。种系发生树分析表明,RcDREB1与小麦AP2家族DREB类基因高度同源。酵母单杂交试验表明,RcDREB1表达的蛋白具有特异结合干旱应答DRE元件的功能;通过实时定量PCR分析发现,高盐、干旱、重金属镉和低温胁迫均可诱导RcDREB1表达,但其对高盐反应较为显著。本研究表明,RcDREB1是DREB类转录因子基因,在介导植物响应逆境信号方面可能发挥着重要生物学功能,为进一步分析小麦族的进化和转基因应用奠定了基础。

    Abstract:

    In this paper, a full-length cDNA of a DREB-like gene was cloned firstly from Roegneria ciliaris through RT-PCR and RACE technologies, and named as RcDREB1. RcDREB1 contains a conserved AP2 domain, suggesting that this gene is a new transcription factor member in the AP2 superfamily. Phylogenetic tree analysis showed that RcDREB1 shares a high similarity with the DREB-like genes in Triticum aestivum. Through yeast one-hybrid experiment, RcDREB1 was verified to possess an ability to specifically bind the drought-responsive DRE element; Using Real-time quantitative PCR, we found that all of the stresses of high salt, drought, heavy metal (CdCl2) and low temperature could up-regulate RcDREB1 expression, but only high salt showed more strong induction of RcDREB1. These results suggested that RcDREB1 is a novel DREB type transcription factor gene and may play important roles in mediating the transduction of distinct signaling cues initiated by those of adverse environmental factors. This DREB-like gene could be useful in elucidating the evolution pathway of Triticeae and in the application in transgenic wheat breeding.

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赵艳红,朗明林,王小龙,等.纤毛鹅观草DREB类基因RcDREB1的克隆及其蛋白结合干旱应答元件DRE的功能验证[J].植物遗传资源学报,2013,14(5):857-863.

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  • 收稿日期:2013-01-30
  • 最后修改日期:2013-04-19
  • 录用日期:2013-08-21
  • 在线发布日期: 2013-08-21
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