In order to excavated the excellent tea germplasms, 51 accessions tea landraces were taken as materials from Yunnan province, and the genetic diversity was investigated based on agronomic and quality traits. The results showed those accessions had the rich genetic variation in agronomic and quality traits. The average variation coefficient and diversity index of agronomic characters was 25.84% and 1.94. The average variation coefficient and diversity index of biochemical composition was 16.53% and 1.88 . Based on sensory evaluation, the total score of black tea was 87.5 to 94.2 points, and green tea was 78.8 to 91.5 points. Their diversity index of adaptability was 0.92 and 0.94. Based on agronomic and quality traits, 51 materials were clustered into 3 groups: the groupⅠ suited to be processed black tea, the groupⅡ suited to be processed green tea, the group Ⅲ was the special varieties on some biochemical composition. 18 fine varieties were selected and used for future production and breeding.