新型抗除草剂棉花不育系Yu98-8A1的培育及鉴定
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国家高技术研究发展计划(863计划)


Cultivation and Identification of Herbicide-resistant Genic Male-sterile line Yu98-8A1 of cotton(Gossypium hirsutum L.)
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The National High Technology Research and Development Program of China (863 Program)

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    摘要:

    通过连续回交,将抗除草剂基因EPSPS-G6转育花粉败育彻底(无微量花粉,不育度达100%)的棉花单基因隐性控制的核不育系Yu98-8A,进而培育成抗除草剂核不育系Yu98-8A1。对该转育不育系花冠表型测量观察表明,与同质系正常可育株比较,不育株花冠较小, 不育株子房直径略大于可育株,花柱长和花柱外露长度均明显高于同质系正常可育株,花柱头外露为其最显著的表型特征;显微观察显示,不育系Yu98-8A1小孢子败育主要是在四分体形成后的小孢子发育期。小孢子败育特征表现为花粉粒无内含物、无刺突产生,最后解体、退化。PCR分子鉴定表明,抗除草剂基因EPSPS-G6转育入Yu98-8A1,除草剂抗性试验表明,该转育不育系可抗质量百分比浓度达0.3%的草甘膦。该抗除草剂核不育系的培育在棉花杂种优势利用方面有重大利用价值。

    Abstract:

    Yu98-8A1, a herbicide-resistant cotton genic male-sterile line, was developed by successive backcrossing cotton line EHC09-002 which has the herbicide-resistant gene EPSPS-G6 to a genic male-sterile line Yu98-8A. Phenotype observation of corrala showed that, compared with homogeneity normal fertile plants, smaller corolla and exposed stigmas were the significant characteristics. Cytological microscopical observation showed that the anther abortion of Yu98-8A1 occurred during the stage of microspore development after tetrad formation, which included that there was no spinescent protuberance on the pollen wall, and the microspore appeared abnormal and break down gradually. PCR identification and glyphosate resistance test indicated that herbicide-resistant gene EPSPS-G6 had been aggregated successfully into Yu98-8A1, and the anti-herbicide concentration reached up to 0.3%. Herbicide resistance makes Yu98-8A1 have significant value in the utilization of cotton heterosis.

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杨晓杰.新型抗除草剂棉花不育系Yu98-8A1的培育及鉴定[J].植物遗传资源学报,2013,14(4):723-727.

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  • 收稿日期:2012-10-14
  • 最后修改日期:2013-01-21
  • 录用日期:2013-06-07
  • 在线发布日期: 2013-06-07
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