省沽油群体遗传多样性的AFLP分析
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Populations Genetic Diversity Analysis of Staphylea bumalda Based on AFLP Markers
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    摘要:

    利用4对AFLP引物对来自安徽石台、湖北大悟和河南桐柏的3个省沽油群体进行遗传多样性分析,共扩增出489条带,多态性条带460条,多态性百分比为93.99%。不同群体Nei’s基因多样性指数(H)和Shannon’s信息指数(I)变化范围分别为0.1920-0.2046和0.2937-0.3151,其中湖北大悟群体遗传多样性最高。物种水平和种群水平的H分别为0.2190和0.1964,群体内变异占总变异的89.68%,表明省沽油遗传变异主要存在于各群体内部。3个群体的平均遗传距离为0.0292,UPGMA聚类分析结果说明省沽油各群体间亲缘关系较近并和地域具有相关性。建议省沽油的遗传资源保护应以种内遗传多样性的保护为主。

    Abstract:

    Genetic diversity of 3 populations of Staphylea bumalda that sampled from Hubei, Anhui and Henan was detected by employing amplified fragment length polymorphism (AFLP) markers. 489 clear bands were amplified with 4 pairs of primer and 460 (93.99%) of them were polymorphic. Nei’s gene diversity and Shannon’s information index ranged from 0.1920 to 0.2046 and from 0.2937 to 0.3151, respectively. The highest population genetic diversity was Dawu from Hubei Province. Nei’s gene diversity for the species and population were 0.2190 and 0.1964, respectively. The analysis of variance showed that the main variance within populations was 89.68%. The mean genetic distance among the 3 population was 0.0292. The results of UPGMA cluster analysis based on genetic distance revealed there was high genetic similarity between the population and the genetic distance was closely related to its distribution area. The research suggests that the important way for Staphylea bumalda conservation will be conserving the high genetic diversity populations.

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李凤鸣,张华新,杨秀艳.省沽油群体遗传多样性的AFLP分析[J].植物遗传资源学报,2012,13(1):130-133.

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