基于染色体片段导入系发掘抗玉米丝黑穗病主效QTL
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家高技术研究发展计划(863计划)


Identification of Major QTL for Head Smut Resistance Based on Donor Chromosome Segment Introgression line and Linkage disequilibrium Analysis in Maize
Author:
Affiliation:

Fund Project:

The National High Technology Research and Development Program of China (863 Program)

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    选用抗玉米丝黑穗病自交系Mo17和SH15为供体,与受体感病自交系黄早四和昌7-2构建回交群体(BC3F1 \BC4F2),通过田间人工接种玉米丝黑穗病原菌鉴定抗病性表现,评价群体抗病性。研究结果显示黄早四×(黄早四×Mo17)BC4F2群体发病率明显高于BC3F1群体;两个BC4F2黄早四×(黄早四×Mo17)和昌7-2×(昌7-2×SH15)群体的发病率差异较大。采用SSR标记分析抗病株的供体染色体导入片段,发现随着回交次数的增多,导入片段数量减少,但不同回交群体中供体导入片段数目明显不同。通过连锁不平衡分析,在染色体2.09和3.04区段发掘和验证2个抗玉米丝黑穗病主效QTL,连锁标记分别为umc2077和phio53或bnlg1965。本文研究结果为抗丝黑穗病基因精细定位和分子聚合育种提供了信息和材料。

    Abstract:

    Mo17 and SH15, two head smut resistant inbred lines as donor parents were chosen to produce BC3F1 and BC4F2 populations of Huangzao4 ×(Huangzao4×Mo17) and Chang7-2 ×(Chang7-2×SH15) with two susceptible inbred lines Huangzao4 and Chang7-2 as recurrent parents, respectively. These populations were evaluated for head smut resistance with artificial inoculation under field condition. The disease incidence for BC4F2 populations of Huangzao4 ×(Huangzao4×Mo17) was higher than that of BC3F1 population. The disease incidence was found different for the BC4F2 populations of Huangzao4 ×(Huangzao4×Mo17) and Chang7-2 ×(Chang7-2×SH15). The resistant individuals were genotyped by SSR markers on maize bins 2.09 and 3.04. The number of donor introgrssion segments in BC4F2 resistant plants was becoming less than that in BC3F1, and also was different in different populations. Using linkage disequilibrium analysis, two major QTL for head smut resistance were identified, each on bin 2.09 and bin 3.04, with linked markers umc2077 and phio53\bnlg1965, respectively. The results obtained provided both useful genetic information and materials for QTL mapping and marker assisted selection for head smut resistance in maize.

    参考文献
    相似文献
    引证文献
引用本文

吉海莲,翁建峰,吕香玲,等.基于染色体片段导入系发掘抗玉米丝黑穗病主效QTL[J].植物遗传资源学报,2012,13(2):244-251.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2011-07-07
  • 最后修改日期:2011-09-02
  • 录用日期:2011-12-28
  • 在线发布日期: 2012-02-07
  • 出版日期:
您是第位访问者
ICP:京ICP备09069690号-23
京ICP备09069690号-23
植物遗传资源学报 ® 2024 版权所有
技术支持:北京勤云科技发展有限公司