香蕉离体茎尖超低温保存研究
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“十一五”国家科技支撑计划(2006BA013B10);中央级公益性科研院所基本科研业务费专项资助项目(082060302-07);农业部农作物种质资源保护利用专项资助项目(NB08-2130135)


Cryopreservation of in Vitro Shoot-Tips of Banana(Musa spp.)
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    摘要:

    以香蕉(Musa spp.)试管苗为试材,对其离体茎尖小滴玻璃化法超低温保存的影响因素进行了研究。小滴玻璃化法和玻璃化法超低温保存后再生率的差异表明,香蕉更适合用小滴玻璃化法进行超低温保存。香蕉小滴玻璃化法超低温保存的方案如下:试管苗在60g/L蔗糖的MS培养基上培养1~2个月,剥离带有1~2片叶原基的茎尖,室温下装载30min(可延长至4h),0℃下PVS2处理40~50min。6个基因型的14个品种的再生率平均为46.9%。通过SSR分子标记检测,再生植株的遗传稳定性没有发生改变。该结果为香蕉种质资源的长期保存提供了理论依据和技术支撑。

    Abstract:

    In vitro shoot-tips of banana(Musa spp.)were successfully cryopreserved by droplet vitrification method.The factors affecting the post-thaw regeneration of shoot-tips were analyzed.The results showed that the droplet vitrification method was more suitable for cryopreservation of banana in vitro shoot-tips than vitrification method.The optimal procedures were as follows:Shoot-tips with 1 or 2 leaf primordia excised from rooted in vitro plantlets which were cultured on MS medium supplemented with 60g/L sucrose for 1~2 months.30min loading time could be prolonged up to 4h at room temperature,and the optimal treatment time of PVS2 was 40~50min at 0℃.The average post-thaw regeneration rate was 46.9% of 14 accessions belonging to six different genomic groups of Musa spp.after cryopreservation by droplet vitrification method.There was no variation in the regenerated plantlets on SSR molecular level

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李俊慧,何平,陈晓玲,等.香蕉离体茎尖超低温保存研究[J].植物遗传资源学报,2010,11(1):34-39.

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