Selecting stable resistant germplasm is an important foundation of breeding diseaseresiatance varieties.In this paper,the bacterial wilt(BW)resiatance performance of fortyeight tobacco germplasm were identified by inoculation method and field disease nursery method.Soil potted inoculation results showed that seven germplasm(CF207,Yanyan 97,TI448A,DB101,G80,RG17 and GTH-1)were of high resistance,and nine germplasm(MSK149,Oxford 2028,NC95,YN108,K346,K358,Enshu FC,Oxford 207 and RG11)were of resistance.Float seedling water inoculation results showed that Oxford207,Yanyan 97 were of high resistance,and Enshu FC were of resistance.Their F1 hybrids with Yunyan 85 and K326 were of resistance to medium susceptible.In field district test,four approved varieties(RG11,RG17,K358 and K346)showed medium resistance to BW and high output;TI448A showed resistance to BW and susceptible to black shank and hollow stalk;Oxford 2028 and Oxford 207 showed resistance or high resistance to BW and high output.G3 and Yanyan 97 showed high resistance,resistance respectively and low output.According to seedling period and two years field identification results,four germplasm(Oxford 207,Enshu FC,Yanyan 97 and TI448A)have great breeding potential as bacterial wilt resistance resource.