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  • 王珊,蔡斌华,章镇,侯计华,徐军霞,高志红.果梅完全花与不完全花的差异蛋白分析[J].植物遗传资源学报,2008,(4):.    [点击复制]
  • .Analysis of Differential Proteins in Perfect and Imperfect Flowers of Japanese Apricot(Prunus mume Sieb.et Zucc.)[J].植物遗传资源学报,2008,(4):.   [点击复制]
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果梅完全花与不完全花的差异蛋白分析
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摘要:
应用双向电泳技术对果梅完全花与不完全花的蛋白质组分进行了比较分析。经专业分析软件(PDQuest)对电泳图谱分析表明两者的蛋白分布相似,在完全花中发现了1个特异蛋白、1个上调蛋白、21个下调蛋白,在不完全花中发现2个特异蛋白,这些蛋白差异点可能与雌蕊的败育有关。应用质谱技术对3个特异点及5个差异大的蛋白点进行分析,得到的肽段数据与蛋白质数据库比对发现其中一个蛋白(28.2kD,pI4.53)与光敏色素B有关。
关键词:  果梅  完全花  不完全花  差异蛋白  肽质量指纹谱
DOI:
基金项目:
Analysis of Differential Proteins in Perfect and Imperfect Flowers of Japanese Apricot(Prunus mume Sieb.et Zucc.)
Abstract:
Two-dimensional electrophoresis was used to analyze differential proteins between the perfect and imperfect flowers in Japanese apricot(Prunus mume Sieb.et Zucc).About 400 proteins were detected after analysis with software(PDQuest).One specific protein,one up-regulated proteins and twenty-one down-regulated proteins were found in the perfect flowers,two specific protein were found in the imperfect flowers,which might be related to the development of pistil.Three specific protein and five differential proteins were identified by mass spectrometry.And identified proteins were presented through the protein database,No.A7(28.2kD,pI 4.53)was related to the phytochrome B.
Key words:  Japanese apricot(Prunus mume Sieb.et Zucc),Perfect flowers,Imperfect flowers,Differential proteins,Peptide mass fingerprinting

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