1.西北农林科技大学园艺学院;2.西北农林科技大学林学院;3.榆林市林业产业开发中心
国家自然科学基金项目(面上项目,重点项目,重大项目)
1.Horticultural Science Research Center,College of Horticulture,Northwest A F University;2.Jujube engineer technique research center of National Forestry and Grassland Administration,College of Forestry,Northwest A F University;3.Yulin Forestry Industry Development Center
The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)
以陕北清涧76份野生酸枣种质为材料,对其果实表型、营养品质及种仁药用成分等性状指标进行多样性、主成分及灰色关联度等分析,旨在筛选优良种质,为野生酸枣种质资源保护及挖掘特异种质提供参考。结果表明:15个果实表型性状变异丰富,变异系数在7.04%~47.86%之间,其中单果质量与单核质量的变异程度最高,而可食率的变异系数最低为7.04%;果核横径、果核纵径的Shannon-Weaver多样性指数(H′)最高,分别达1.867和1.838。5个果实营养品质性状变异系数在32.39%~89.70%范围,其中糖酸比的变异系数达89.70%;可滴定酸、可溶性固形物及维生素C含量的遗传多样性指数均大于1.5。基于高效液相色谱法(HPLC)成功建立了供试61份野生酸枣种仁药用成分的指纹图谱,并对供试76份野生酸枣药用成分含量进行测定,发现不同种质药用成分含量存在显著差异,其中种仁皂苷B的变异系数最大达68.16%,皂苷A的多样性指数H'最高为1.374。以上结果表明,供试野生酸枣种质在果实品质及药用成分性状均具有丰富的遗传多样性。通过主成分分析提取了7个主因子,累计贡献率72.59%,分别反映果实形态、营养品质、种仁形态及药用成分等核心指标,并利用熵权法结合灰色关联法综合评价,分别筛选出性状优良的鲜食和种仁药用的野生酸枣种质资源各3份,为酸枣鲜食选育及种仁药用资源开发提供优良种质。
The study utilized 76 wild sour jujube (Ziziphus jujuba var. spinosa) germplasm resources from Qingjian, northern Shaanxi, as experimental materials. Fruit external phenotypes, nutritional quality traits and kernel medicinal components were analyzed for genetic diversity, correlation, principal components and Grey Relational Analysis(GRA), aiming to select superior individuals and provide a reference for the conservation of wild sour jujube germplasm and the mining of exceptional accessions.The results showed that the 15 quantitative fruit phenotypic traits were highly variable, with coefficients of variation (CV) ranging from 7.04 % to 47.86 %. Single-fruit mass and single-stone mass displayed the greatest variation, whereas the coefficients of variation for edible-rate was the lowest (7.04 %). Stone width and stone length exhibited the highest Shannon–Weaver diversity indices(H′), reaching 1.867 and 1.838, respectively. Among the five fruit nutritional traits, the sugar-acid ratio showed the highest CV to 89.70 %, and the H′ indices of titratable acid, soluble solids and vitamin C all exceeded 1.50, indicating a high level of genetic diversity. High-performance liquid chromatography (HPLC) fingerprints of kernel Pharmacodynamic components were successfully established for 61 Qingjian sour jujube resources. Four pharmacodynamic compounds were quantified, revealing significant differences among individual plants. Jujuboside B exhibited the greatest variation (CV=68.16 %), whereas jujuboside A registered the highest H′ index (1.374). These findings demonstrate that both fruit nutritional quality and kernel medicinal composition in the tested wild sour jujube vary widely and display abundant diversity. Principal-component analysis condensed the 24 fruit related traits into seven factors that collectively explained 72.59 % of the total variance, representing core aspects of fruit morphology, nutritional quality, kernel morphology and medicinal constituents. Using a combination of the entropy weight method and grey relational analysis(GRA) for comprehensive evaluation, three wild jujube germplasm resources with excellent comprehensive traits were screened for fresh consumption and three for seed kernel medicinal use, respectively. This provides high-quality germplasm for fresh-fruit breeding programmes and the development of kernel pharmacodynamic sour jujube resources.
