The initial tendril node is a crucial agronomic trait in cucumber，serving as a key indicator of the plant's transition from the seedling stage to the vine-growing stage. However，no genes regulating the initial tendril node in cucumber have been reported to date. This study used 229 cucumber core germplasm accessions from four different ecotypes (European, Pickled, North of China, and South of China) as experimental materials. The initial tendril node was investigated in plastic greenhouses at the Shouguang experimental base of the Chinese Academy of Agricultural Sciences in April and August 2024. Phenotypic analysis was conducted, and through genome-wide association analysis, four repeated loci were detected on Chr.4, Chr.5, and Chr.6, respectively: gITN4.1, gITN5.1, gITN6.1, and gITN6.2. Based on gene sequence analysis，haplotype，and gene expression level analysis at these four loci，six candidate genes associated with tendril initiation node were identified：CsaV3_4G010490，CsaV3_4G010540，CsaV3_5G025510，CsaV3_5G025520，CsaV3_6G037390 and CsaV3_6G051770. These findings providing valuable germplasm resources for breeding cucumber varieties suited to simplified cultivation practices. This work also lays the foundation for elucidating the molecular regulatory mechanisms governing the initial tendril nodes in cucumber.