Seed shattering in plants is an important survival strategy formed during the process of species evolution and is prevalent in nature. It helps plants better adapt to the surrounding environment by promoting seed dispersal and reproduction. However， in agricultural production aimed at seed production， the seed shattering trait has become a key factor restricting production efficiency. Excessive seed shattering leads to massive grain loss in the field and thus causes yield reduction， while insufficient seed shattering increases harvesting difficulty and costs. Neither of these two traits meets the requirements of large-scale seed production. To address this issue， based on years of practical experience and research accumulation in rice shattering identification， this paper systematically sorts out the technical methods and operational procedures for shattering identification， develops an identification technical protocol covering the collection of rice panicle at the mature stage， identification instruments， identification steps， grading and evaluation criteria for rice shattering traits，clarifies the technical specifications for identification operations， and aims to provide standardized technical support for the precision identification of rice shattering trait in rice germplasm resources and related breeding practices. This protocol is applicable to the identification and evaluation of rice shattering trait in mature panicles of rice （Oryza sativa L）.