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首页 > 过刊浏览>2026年第27卷第2期 >338-350. DOI:10.13430/j.cnki.jpgr.20250901001 优先出版
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79份芥蓝材料种子萌发期耐盐碱筛选与评价
DOI:
10.13430/j.cnki.jpgr.20250901001
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作者:
作者单位:

河北北方学院农林科技学院,张家口 075132

作者简介:

研究方向为蔬菜遗传育种,E-mail: 17320780003@163.com

通讯作者:

戴希尧, 主要研究方向为蔬菜抗逆育种,E-mail: daixiyao@163.com

中图分类号:

基金项目:

河北省高等学校科学技术研究项目(Z2020208);河北省大学生创新训练计划项目(S202510092030);河北北方学院研究生案例库建设项目(ALK202508)


Screening and Evaluation of 79 Chinese Kale Materials for Salt-alkali Tolerance During Seed Germination Period
Author:
Affiliation:

College of Agriculture and Forestry Science,Hebei North University, Zhangjiakou 075132

Fund Project:

Foundation projects: Science and Technology Research Project of Higher Education Institutions in Hebei Province (Z2020208);Innovation Training Program for College Students in Hebei Province (S202510092030);Case Database Construction Project for Graduate Students at Hebei North University (ALK202508)

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    摘要:

    为评价79份芥蓝材料萌发期的耐盐碱性并进行分类,测定了对照(去离子水,pH 6.5)、盐胁迫(NaCl,Na+浓度为240 mmol/L,pH 6.5)和盐碱胁迫(NaCl+Na2CO3,Na+浓度为240 mmol/L,pH 9.0)3个处理的发芽势、发芽率、发芽指数、芽鲜重、活力指数、叶绿素含量、根长和芽长8项指标,并进行主成分分析、隶属函数分析、综合耐盐碱指数D值和聚类分析。结果表明,与对照相比,盐胁迫及盐碱胁迫对各项萌发指标均表现出抑制作用,其中对根长的抑制幅度最大,分别下降92.08%和91.49%,变异系数分别增加5.36倍和6.36倍;盐碱胁迫与盐胁迫相比,不同萌发指标显著上升和下降的品种占比不同,其中发芽势显著上升和下降的品种分别占44.30%和24.05%,根长显著上升和下降的品种各占11.39%和5.06%。基于综合耐盐碱指数D值进行聚类分析,79份芥蓝材料分为高度盐(盐碱)敏感型、中度盐(盐碱)敏感型、耐盐(盐碱)型、中度耐盐(盐碱)型和高度耐盐(盐碱)型5类,种质份数依次为13(14)份、19(12)份、21(27)份、19(17)份和7(9)份。通过极端型材料的维恩图分析,筛选出高度双重盐-盐碱敏感型材料12份和高度双重耐盐-盐碱型材料6份;随机抽取4份高度双重盐-盐碱敏感型和4份高度双重耐盐-盐碱型材料的8项相对指标比较表明,除相对芽鲜重和相对根长外,高度双重耐盐-盐碱型品种的发芽势、发芽率等6项相对指标均显著或极显著高于高度双重盐-盐碱敏感型品种。本研究结果可为芥蓝耐盐碱品种选育及关键抗逆基因挖掘提供重要资源基础。

    Abstract:

    To evaluate and classify the salt-alkali tolerance of 79 Chinese kale samples during the germination stage, this study examined eight indicators including germination potential, germination rate, germination index, shoot fresh weight, vigor index, chlorophyll content, root length, and shoot length under three treatments: control check ( deionized water, pH 6.5), salt stress ( NaCl , Na+ 240 mmol/L , pH 6.5), and salt-alkali stress (NaCl + Na?CO?, Na+ 240 mmol/L, pH 9.0). Principal component analysis, membership function analysis, comprehensive salt-alkali tolerance index D-value, and cluster analysis were conducted. The results showed that, compared with control check , both salt stress and salt-alkali stress inhibited all germination indicators, with the most significant suppression observed in root length which decreased by 92.08% and 91.49%, respectively, and the coefficient of variation increased by 5.36 and 6.36 times. When comparing saline-alkali stress with salt stress, the proportions of varieties showing significant increases and decreases in different germination indicators varied. For germination potential, 44.30% of varieties showed a significant increase, while 24.05% showed a significant decrease. Meanwhile, 11.39% of varieties showed a significant increase, and 5.06% showed a significant decrease for root length. Based on the cluster analysis of the comprehensive salt-alkali tolerance index D-value, Chinese kale materials were classified into five categories: highly salt (salt-alkali) sensitive type, moderately salt (salt-alkali) sensitive type, salt (salt-alkali) tolerant type, moderately salt (salt-alkali) tolerant type, and highly salt (salt-alkali) tolerant type, with the number of varieties in sequence is 13 (14), 19 (12), 21 (27), 19 (17), and 7 (9), respectively. Venn diagram analysis of extreme-type materials identified 12 highly double salt-salt-alkali sensitive types and six highly double salt-salt-alkali tolerant types. A comparison of eight relative indicators of four randomly selected highly double salt-salt-alkali sensitive type and four highly double salt-salt-alkali tolerant type materials showed that, except for relative shoot fresh weight and relative root length, the other six relative indicators of the highly double salt-salt-alkali tolerant type were significantly or extremely significantly higher than those of the highly double salt-salt-alkali sensitive type. The results of this study provide an important resource foundation for the breeding of salt-alkali tolerant Chinese kale varieties and the identification of key stress-resistant genes.

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李素亚,董雪霏,郑林洋,等.79份芥蓝材料种子萌发期耐盐碱筛选与评价[J].植物遗传资源学报,2026,27(2):338-350.

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  • 收稿日期:2025-09-01
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  • 在线发布日期: 2026-02-04
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