186份甘蓝型油菜种子萌发期耐盐种质资源鉴定
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1.湖南科技大学生命科学与健康学院;2.岳麓山实验室

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湖南省农业科技创新资金项目(2022年度)《耐盐碱油菜种质资源筛选与育种利用》;湖南省科技创新计划资助;湖南省科技人才托举工程项目


Identification of Salt-Tolerant Germplasm During Seed Germination Period in 186 accession of Brassica napus
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The agricultural technology innovation fund project of Hunan Province (2022)《Screening and breeding utilization of salt-alkaline tolerant rapeseed germplasm resources》;The science and technology innovation program of Hunan Province;The science and technology talent support program of Hunan Province

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    摘要:

    盐胁迫对植物的生长产生严重抑制作用,导致作物产量降低,萌发期是植物对盐害最为敏感的时期。因此建立准确便捷的耐盐性鉴定评价体系和筛选耐盐甘蓝型油菜种质对提高油菜品种抗盐能力至关重要。本研究根据15份甘蓝型油菜种质的盐损伤指数筛选出萌发期耐盐性鉴定的最佳浓度为1.25%,在该浓度下对186份甘蓝型油菜种质资源的萌发势、萌发率、根长、下胚轴长、鲜重和干重进行测定,并计算其耐盐指数。通过主成分分析、隶属函数等分析方法对油菜种质资源进行耐盐性综合评价,并建立耐盐性综合评价模型,筛选出适宜的评价鉴定指标。结果表明:1.25% NaCl胁迫下,甘蓝型油菜的相对萌发势、相对萌发率、相对根长、相对下胚轴长、相对鲜重、相对干重间互成极显著正相关;利用主成分分析将6项指标综合为3个主成分,累计贡献率达92.809%,依据3个主成分的权重计算各种质的D值,将186份种质划分为4个等级,筛选出5份耐盐材料。并建立了甘蓝型油菜耐盐性鉴定的数学评价模型D=0.111+0.201X1+0.165X2+0.381X5。本研究筛选得到的耐盐鉴定指标和耐盐材料为开展油菜耐盐育种奠定了方法和材料基础。

    Abstract:

    Salt stress severely inhibits the growth of plants, resulting in a decrease in crop yield. The germination period is the most sensitive phase for plants to salt damage. Therefore, establishing an accurate and convenient salt tolerance identification and evaluation system, as well as screening for salt-tolerant germplasm in Brassica napus, is crucial for enhancing the salt resistance of rapeseed varieties. In this study, the optimal concentration for salt tolerance identification during the germination period was determined to be 1.25% based on the salt damage index of 15 Brassica napus germplasms. At this concentration, the germination vigor, germination rate, root length, hypocotyl length, fresh weight, and dry weight of 186 Brassica napus germplasm resources were measured, and their salt tolerance indices were calculated. Conducting comprehensive salt tolerance assessment of rapeseed germplasm resources using analysis methods such as principal component analysis and membership functions. A salt tolerance comprehensive evaluation model was established, and suitable evaluation indicators were selected. The results showed that under 1.25% NaCl stress, there was a highly significant positive correlation among the relative germination vigor, relative germination rate, relative root length, relative hypocotyl length, relative fresh weight, and relative dry weight of Brassica napus. By employing principal component analysis, these six indicators were condensed into three principal components, which collectively account for a cumulative variance of 92.809%. Based on the weights of these three principal components, D values were calculated for various germplasms, resulting in the classification of 186 germplasms into four levels and the identification of five salt-tolerant materials. Furthermore, a mathematical evaluation model for salt tolerance identification in Brassica napus was established as D=0.111+0.201X1+0.165X2+0.381X5. The salt tolerance identification criteria and salt-tolerant materials chosen in this study have established the methodological and material groundwork for salt-tolerant rapeseed breeding.

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  • 收稿日期:2024-02-23
  • 最后修改日期:2024-05-17
  • 录用日期:2024-09-02
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