In this study, two peanut varieties, red pearl (H) and white pearl (B, the control), were used as research samples for transcriptomic-metabolomics combined analysis. For the detection of testa color ( L- , a- and b-value) and anthocyanin content in DAF 30 and DAF 45，sample H and B showed significant differences between varieties (P<0.05). FPKM hierarchical cluster analysis showed that there were 402 identical differential expressed genes (DEGs) in 4 comparison groups, and there were 173, 1 017, 1 847 and 1 843 unique genes, respectively.GO analysis annotation results showed there were 8 GO Terms significantly related to anthocyanin synthesis. Among them, GO:0055114 and GO:0016207 had enriched with 8 and 7 DEGs respectively. The results of KEGG enrichment analysis showed that 6 metabolic pathways were significantly related to anthocyanin biosynthesis, among which flavonoids biosynthesis pathway enriched 6, 17, 15 and 23 DEGs, and the enrichment factors were 2.17, 2.10, 1.25 and 2.62, respectively. Metabolomics results showed that Cyanidin, Procyanidin, Petunidin, Delphinidin, Malvidin, peony (Peonidin) and their derivatives were located in differential accumulated metabolites (DAMs). The transcriptomics-metabolomics combined analysis showed that flavonoid biosynthesis (ko00941) is the key synthetic pathway , and delphin and centaurea are the key DAMs of testa color formation. were verified by qRT-PCR. The expression trend of 11 detected DEGs was consistent with the results of transcriptome sequencing. The results of this study have a certain reference significance for revealing the regulatory mechanism of anthocyanin synthesis in peanut testa.