1.河北农业大学农学院/华北作物种质资源教育部重点实验室/河北种质资源实验室,保定 071001;2.濮阳市农林科学院,河南濮阳 457000;3.承德市农林科学院,河北承德 067000
研究方向为植物种质资源创制与利用,E-mail:2734182026@qq.com
穆国俊,研究方向为花生种质资源创制与利用,E-mail: mgj99999@126.com
杨鑫雷,研究方向为花生遗传与分子育种, E-mail:peanut@hebau.edu.cn
河北省现代农业产业技术体系建设专项资金(HBCT2024040201);河北省高等学校科学技术研究项目(ZD2022069);河北省青年拔尖人才资助项目(0602015);河北省重点研发计划项目现代种业科技专项(19226363D);曲阳庄子河太行山农业创新驿站建设项目(903-311718001)
1.Agronomy College, Hebei Agricultural University/North China Key Laboratory for Crop Germplasm Resources of Education Ministry/Hebei Germplasm Resources Laboratory, Baoding, 071000;2.Puyang Academy of Agricultural and Forestry Sciences, Puyang 457000,Henan;3.Chengde Academy of Agriculture and Forestry Sciences, Chengde 067000, Hebei
Foundation projects: Hebei Province Modern Agricultural Industrial Technology System Construction Special Fund(HBCT2024040201); Science and Technology Research Project of Colleges and Universities in Hebei Province(ZD2022069); Project of Youth Top Talent Funding in Hebei Province(0602015); Key Project of Science and Technology Research of Modern Seed Industry of the Department of Science and Technology in Hebei Province(19226363D); Project of the Construction of Zhuangzi River Taihang Mountain Agricultural Innovation Station in Quyang(903-311718001)
花生是我国重要的特色出口农产品,在农业发展中占有至关重要的地位。本研究以红珍珠(H)和白珍珠(B)两个花生品种为研究材料,进行转录组学-代谢组学联合分析。在开花后第30天和第45天,红珍珠种皮和白珍珠种皮色差值(L值、a值、b值)及其花青素含量在品种间均表现极显著差异。FPKM层次聚类分析结果表明,开花后第30天红珍珠相对于开花后第30天白珍珠和开花后第45天红珍珠相对于开花后第45天白珍珠独有基因分别为1847和1843个。GO分析注释结果表明, 8条GO 通路与花青素合成密切相关,其中GO:0055114和GO:0016207两个条目分别富集到8个和7个差异表达基因。KEGG富集分析结果表明,6条代谢途径与花青素生物合成显著相关。代谢组学结果表明,差异代谢物定位到了矢车菊素、原花青素、矮牵牛素、翠雀花素、锦葵素、牡丹素及其衍生物。转录组-代谢组联合分析结果表明,类黄酮生物合成途径(ko00941)是种皮颜色形成的关键途径,翠雀花素和矢车菊素为关键差异代谢物。11个差异表达基因qRT-PCR表达趋势基本与转录组测序结果一致。本研究结果对揭示花生种皮花青素生物合成调控机制具有一定的参考意义。
Peanut is the important unique export agricultural product, which occupies a vital position in agricultural development of China.In this study, two peanut varieties, Hongzhenzhu (H) and Baizhenzhu (B, the control), were used as research samples for transcriptomic-metabolomics combined analysis. At 30 and 45 days after flowering, the testa color (L value, a value, b value) and anthocyanin content of Hongzhenzhu and Baizhenzhu were extremely significantly different between varieties. FPKM hierarchical cluster analysis showed that compared with Baizhenzhu, there were 1847 and 1843 unique genes at 30 and 45 days after flowering in Hongzhenzhu, respectively. GO analysis annotation results showed there were 8 GO terms significantly related to anthocyanin synthesis. Among them, GO:0055114 and GO:0016207 had enriched with 8 (C4H、two CHS、F3′H、two FLS、F3H and PAL)and 7(two CHS、CHI、F3′H、two FLS and F3H) differential expressed geness respectively. The results of KEGG enrichment analysis showed that 6 metabolic pathways were significantly related to anthocyanin biosynthesis, respectively. Metabolomics results showed that cyanidin, procyanidin, petunidin, delphinidin, malvidin, peony (peonidin) and their derivatives were differential accumulated metabolites (DAMs). The transcriptomics-metabolomics combined analysis showed that flavonoid biosynthesis (ko00941) is the key synthetic pathway and delphin and centaurea are the key DAMs of testa color formation. The qRT-PCR result of 11 detected DEGs was consistent with the results of transcriptome sequencing. These results of this study have a certain reference significance for revealing the regulatory mechanism of anthocyanin synthesis in peanut testa.
张利苹,王俊玲,李振华,等.花生红色种皮花青素生物合成转录-代谢组学联合分析[J].植物遗传资源学报,2024,25(10):1767-1780.
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