大豆种质资源苗期耐盐鉴定及遗传多样性分析
作者:
作者单位:

东北农业大学农学院/大豆生物学教育部重点实验室/农业农村部东北大豆生物学与遗传育种重点实验室,哈尔滨 150030

作者简介:

研究方向为大豆遗传育种,E-mail:1520491419@qq.com

通讯作者:

滕卫丽,研究方向为大豆遗传育种与生物技术,E-mail: twlneau@163.com

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基金项目:

国家重点研发计划项目(2021YFD1201103-01-03);财政部、农业农村部现代农业产业技术体系(CARS-04-PS04)


Identification of Salt-tolerant Germplasm Resources in Soybean Seedlings and Genetic Diversity Analysis
Author:
Affiliation:

College of Agriculture, Northeast Agricultural University/Key Laboratory of Soybean Biology in Chinese of Ministry of Education/ Key Laboratory of Soybean Biology and Breeding (Genetics) in Northeast China of the Ministry of Agriculture and Rural Affairs, Harbin 150030

Fund Project:

Foundation projects: National Key Research and Development Project Program(2021YFD1201103-01-03); Modern Agricultural Industrial Technology System,Ministry of Finance, Ministry of Agriculture and Rural Affairs (CARS-04-PS04)

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    摘要:

    盐碱地是边际土壤的主要类型之一,利用边际土地耕作是减缓耕地紧缺的有效途径。为筛选耐盐性较强的大豆种质资源,提高盐碱土地大豆产量,本研究对392份来自国内外不同地域的大豆种质资源,采用150 mmol/ L NaCl进行苗期盐胁迫处理,采用单株分类记载法进行苗期耐盐性鉴定,并利用10个与耐盐基因连锁的SSR标记对高耐及耐盐等级大豆种质资源进行分子辅助鉴定及遗传多样性分析,应用相似性系数分析、聚类分析等方法对高耐及耐盐等级大豆种质资源进行综合评价。结果表明:筛选出58份高耐及耐盐大豆种质资源,包括赤豆1号、东农69等高耐大豆种质资源14份,黑农51、黑河35等耐盐大豆种质资源44份;58份耐盐大豆种质资源分子辅助鉴定表明,绥农1号、合丰50和东大2号携带耐盐等位变异最多,均为6个,标记平均鉴定效率为43.45%,平均准确率为68.46%,其中分子标记Satt201的鉴定效率和鉴定准确率最高,分别为60.34%和96.55%;聚类分析表明,58份大豆种质资源间的相似性系数在0.5385~0.9231之间,平均值为0.6974,相关系数为0.6240,说明58份大豆种质资源大部分遗传关系较近,遗传多样性较低,58份耐盐大豆种质资源并未按地域进行聚类,但一个类群或亚群中大部分种质资源来源地在地理位置上相同或较为接近,可从中筛选亲缘关系较远的大豆种质资源作为亲本,用于培育耐盐大豆新品种奠定遗传基础。

    Abstract:

    Saline-alkali soil is one of the main types of marginal soils. Using the marginal land for agricultural cultivation is an effective way to alleviate the shortage of farming land. In order to screen soybean germplasm resources showing salt tolerance to improve soybean yield in saline soils, 392 samples from different geographic regions at home and abroad were treated with 150 mmol/L NaCl at the seedling stage. Each single plant was identified and genotyped using 10 SSR markers linked to salt tolerant genes, in order to perform molecular-assisted identification and genetic diversity analysis. Similarity coefficient analysis, cluster analysis and other methods were applied to comprehensively evaluate the soybean germplasm resources. Fifty-eight soybean germplasm resources were identified, including 14 showing high tolerance, such as Chidou 1 hao and Dongnong 69, and 44 showing salt-tolerant, such as Heinong 51 and Heihe 35. Although genotyping these 58 samples, Suinong 1 hao, Hefeng 50 and Dongda 2 hao carried the most salt tolerant allele variations, all of which were 6, and the average identification efficiency was 43.45% and the average accuracy was 68.46%, of which the molecular markers Satt201 had the highest identification efficiency of 60.34% and the highest accuracy of 96.55%. Cluster analysis showed that the similarity coefficients among the 58 soybean germplasm resources ranged from 0.5385 to 0.9231, with an average value of 0.6974 and a correlation coefficient of 0.6240, indicating that most of the 58 soybean germplasm resources were genetically close, and the genetic diversity was relatively low, and that the 58 soybean germplasm resources were not clustered geographically, but most of them were geographically identical or had the same place of origin in a taxon or subgroup. However, we didn’t detect the correlation between genetic similarity and geographically collection sites. The distant germplasm resources can be selected as parents, to breed new salt-tolerant soybean varieties.

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引用本文

林峰,赵慧艳,史飞飞,等.大豆种质资源苗期耐盐鉴定及遗传多样性分析[J].植物遗传资源学报,2024,25(6):945-956.

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  • 收稿日期:2023-11-28
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  • 在线发布日期: 2024-06-11
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