模拟干旱胁迫下蜡杨梅幼苗根系响应5-羟色胺的转录组分析
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1.宁波市特色园艺作物品质调控与抗性育种重点实验室;2.江西农业大学农学院;3.慈溪市林特技术推广中心

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宁波市现代种业专项“杨梅种质创新及关键栽培技术研究”


Transcriptomic analysis in Morella cerifera seedlings under simulated drought stress in response to 5-hydroxy-tryptamine
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Ningbo Key Laboratory of Characteristic Horticultural Crops in Quality Adjustment and Resistance Breeding

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The modern seed industry special project in Ningbo

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    摘要:

    植物生长调节剂5-羟色胺(5-HT)已应用于农业生产,以提升作物抗旱性,然而其转录水平的分子机制还不清楚。本研究通过转录组测序、内源激素水平和抗氧化酶活性的综合评价,探讨模拟干旱胁迫下蜡杨梅幼苗响应外源5-羟色胺的生理和分子影响机制。结果表明,50 μM的5-羟色胺处理显著提高了蜡杨梅根系中脱落酸(ABA)和茉莉酸(JA)含量,而100 μM的5-羟色胺处理的结果则刚好相反。50 μM的5-羟色胺处理诱导丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性显著升高,而过氧化氢(H2O2)含量则显著降低。基于上述两种浓度水平的5-羟色胺处理基因集富集分析(GSEA)结果发现,差异表达基因集主要包括抗氧化活性、氧化还原酶活性、生长素和赤霉素介导信号传导、细胞壁生物合成、木聚糖生物合成和果胶代谢等。与抗氧化酶活性及激素代谢相关的差异表达基因主要为PER、LAC、DHAR和PIN等。通过加权基因共表达网络分析(WGCNA)发现8个共表达基因模块与5-羟色胺及干旱胁迫显著相关,其中枢纽基因KAB1218346.1(LOX3)、KAB1219593.1(WRKY53)和KAB1217691.1(CZF1)主要参与激素代谢和转录调控,上述关键基因及其分子调控机制将是今后研究的重要对象。

    Abstract:

    The plant growth regulator 5-hydroxy-tryptamine (5-HT) has been used in agricultural production to improve crop drought resistance. The underlying molecular mechanisms of its transcriptional response are largely unknown. In this study, the simulated drought stress-related physiological and molecular mechanisms of exogenous 5-HT on Morella cerifera seedlings were elucidated, including transcriptome sequencing and the evaluation of endogenous hormone levels and antioxidant enzyme activity. The results demonstrated that abscisic acid (ABA) and jasmonic acid (JA) content in Morella cerifera fibrous roots significantly increased following 50 μM 5-HT treatments but decreased in 100 μM treatments. Both the malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were significantly increased but hydrogen peroxide (H2O2) content decreased following 50 μM 5-HT treatments. Based on gene set enrichment analysis (GSEA), two-concentration levels 5-HT treatments were investigated. The differential gene set terms primarily included antioxidant activity, oxidoreductase activity, auxin and gibberellin-mediated signaling, cell wall biosynthetic, phospholipid translocation, xylan biosynthetic, and pectin metabolic. The differentially expressed genes (DEGs) related to antioxidant enzyme activity and hormone metabolism were mainly PER, LAC, DHAR and PIN. Eight sets of co-expressed gene modules were significantly correlated with 5-HT and drought stress by the weighted gene co-expression network analysis (WGCNA). The hub genes KAB1218346.1(LOX3), KAB1219593.1(WRKY53), KAB1217691.1(CZF1) were primarily associated with hormone metabolism and regulation of transcription. These key genes and their molecular regulatory mechanisms will be important targets for future research.

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  • 收稿日期:2023-11-05
  • 最后修改日期:2024-02-08
  • 录用日期:2024-04-12
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