National Natural Science Foundation of China (32371930); China Postdoctoral Science Foundation (2021M702653); Natural Science Foundation of Hunan Province (2023JJ41041); the Research Foundation of Education Bureau of Hunan Province (22B0281); Changsha Natural Science Foundation (kq2202281)
油茶(Camellia oleifera Abe.)是我国重要木本油料树种，具有重要的经济价值和社会效益。隐花色素（Cryptochrome）是植物蓝光受体之一，参与植物开花调控、光形态建成等生长发育过程。本研究从‘华硕’油茶中克隆到CoCRY1基因，通过生物信息学分析发现，其CDS序列包括2262 bp，编码的蛋白质序列包含684个氨基酸，同源序列及系统发育树分析发现CoCRY1蛋白与茶树(Camellia sinensis) CsCRY1序列相似性最高。组织表达分析显示，CoCRY1的表达量在茎中最高，在花中最低。通过农杆菌转化得到CoCRY1基因异源表达的拟南芥(Arabidopsis thaliana)植株，并且从DNA水平及RNA水平鉴定到阳性植株，对CoCRY1异源表达的拟南芥进行表型分析发现，CoCRY1基因的过量表达导致拟南芥提前开花，且特异性抑制光下胚轴伸长。本研究通过生物信息学，定量分析及异源表达发现，油茶CoCRY1基因在油茶开花等生物学过程中具有重要作用。
Camellia oleifera Abe. is an important woody oil tree in China, which has important economic and social benefits. Cryptochrome is one of the blue light receptors in plants, which is involved in the growth and development of plants such as flowering regulation and photomorphogenesis. In this study, CoCRY1 gene was cloned from C.oleifera ""Huashuo"". Bioinformatics analysis showed that CDS sequence length of CoCRY1 was 2262 bp, and it encoded protein included 684 amino acids. Homologous sequence and phylogenetic tree analysis showed that CoCRY1 protein had the highest similarity with the sequence of tea tree (Camellia sinensis). Tissue expression analysis showed that the expression of CoCRY1 was the highest in stem and the lowest in flower. Arabidopsis thaliana plants with heterogenous expression of CoCRY1 gene were obtained by Agrobacterium transformation, and transgenic plants were identified from DNA and RNA level. Phenotypic analysis of transgenic Arabidopsis showed that overexpression of CoCRY1 gene led to early flowering under long day condition and specifically inhibited the elongation of hypocotyl under light. Through bioinformatics, quantitative analysis and heterologous expression, it was found that CoCRY1 gene plays an important role in the biological process of C.oleifera flowering.