OsGSK2OsGLK1互作调控水稻叶绿素合成与叶绿体发育
作者:
作者单位:

淮阴师范学院生命科学学院,江苏淮安 223300

作者简介:

研究方向为水稻分子育种,E-mail:1847549271@qq.com

通讯作者:

刘 喜,研究方向为水稻遗传育种,E-mail:1240623244@qq.com

中图分类号:

基金项目:

江苏省自然科学基金(BK20191055);江苏高校“青蓝工程”优秀青年骨干教师;江苏省大学生创新创业训练计划重点项目(202210323029Z)


OsGSK2 Interacts with OsGLK1 to Regulate Chlorophyll Synthesis and Chloroplast Development in Rice
Author:
Affiliation:

School of Life Science, Huaiyin Normal University, Huai′an 223300,Jiangsu

Fund Project:

Foundation projects: Natural Science Foundation of Jiangsu Province (BK20191055);Outstanding Backbone Young Teachers of Jiangsu Qinglan Project; Key Projects of Jiangsu Province College Student Innovation Training Program (202210323029Z)

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    摘要:

    叶绿素是水稻光合作用的重要色素,叶绿素的合成决定光合作用的效率,影响植物的产量和品质。在本研究中,发现糖原合成酶激酶OsGSK2过表达植株Go-2成熟期呈现叶片深绿色的表型。相比野生型,Go-2植株叶绿素a、叶绿素b以及类胡萝卜素含量显著升高。透射电镜观察结果显示,相比野生型,Go-2植株叶绿体类囊体片层增多。酵母双杂交“一对一”实验证实OsGSK2与Golden2-Like转录因子OsGLK1存在互作,并进一步通过双分子荧光互补实验确认OsGSK2与OsGLK1存在互作。在水稻原生质体中检测双荧光素酶活性发现,相比单转OsGLK1,OsGSK2与OsGLK1共转显著提高下游靶基因的表达水平。荧光定量PCR结果表明,相比野生型,在Go-2植株中OsGLK1直接调控的靶基因OsPORBOsCAO1LHCB6等转录水平显著上调。研究结果初步揭示了OsGSK2与OsGLK1互作调控水稻叶绿素合成和叶绿体发育的分子机理,进一步拓展了水稻糖原合成酶激酶的分子功能,丰富了水稻叶色调控的分子网络,为水稻高光合分子育种提供了理论依据。

    Abstract:

    Chlorophyll is an important pigment in rice photosynthesis, and its synthesis determines the efficiency of photosynthesis, ultimately affecting plant yield and quality. In this study, it was found that the glycogen synthase kinase OsGSK2 overexpressed Go-2 plants exhibited a dark green leaf phenotype at heading date. Compared to the wild type, the contents of chlorophyll a, chlorophyll b, and carotenoids in Go-2 plants significantly increased. The observation results of transmission electron microscopy showed that compared to the wild type, the chloroplast thylakoid layers of Go-2 plants increased. Yeast two hybrid "one-to-one" experiment confirmed the interaction between OsGSK2 and the Golden2-Like transcription factor OsGLK1, and further confirmed the interaction between OsGSK2 and OsGLK1 through bimolecular fluorescence complementarity experiments. By detecting dual luciferase activity in rice protoplasts, it was found that compared to single transgenic OsGLK1, co transfection of OsGSK2 and OsGLK1 significantly increased the expression level of downstream target genes. The fluorescence quantitative PCR results showed that compared to the wild-type, the transcription levels of target genes (OsPORBOsCAO1LHCB6, etc) directly regulated by OsGLK1 were significantly up-regulated in Go-2 plants. These results provided preliminarily insights on the molecular mechanism of the interaction between OsGSK2 and OsGLK1 in regulating rice chlorophyll synthesis and chloroplast development, extending the molecular function of rice glycogen synthase kinase, enriching the regulation network of rice leaf color, and providing theoretical basis for high photosynthetic molecular breeding in rice.

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引用本文

赵楚瑄,纪晓楠,刘梦雨,等.OsGSK2OsGLK1互作调控水稻叶绿素合成与叶绿体发育[J].植物遗传资源学报,2024,25(3):472-481.

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历史
  • 收稿日期:2023-08-21
  • 最后修改日期:2023-09-02
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  • 在线发布日期: 2024-03-05
  • 出版日期: 2024-02-26
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