Rice is one of the most important food crops in China and even the world, feeding nearly 50% of the world"s population. Chlorophyll is an important pigment in rice photosynthesis, and its synthesis determines the efficiency of photosynthesis, affecting plant yield and quality. In this study, it was found that the glycogen synthase kinase OsGSK2 overexpressed Go-2 plants exhibited a dark green leaf phenotype at heading date. Compared with the wild type, the chlorophyll contents of Go-2 plants were significantly increased, and the thylakoid lamellaes of chloroplasts were increased. Yeast two hybrid and bimolecular fluorescence complementary experiments confirmed that OsGSK2 interacts with the transcription factor OsGLK1. The fluorescence quantitative PCR results showed that the transcription levels of OsGLK1 target genes (OsPORB, OsCAO1, LHCB1, etc) were significantly up-regulated. The research results preliminarily revealed the molecular mechanism of the interaction between OsGSK2 and OsGLK1 in regulating rice chlorophyll synthesis and chloroplast development, further expanding the molecular function of rice glycogen synthase kinase, enriching the molecular network of rice leaf color regulation, and providing important theoretical basis for rice high photosynthetic molecular breeding.