Institute of Crop Sciences,Chinese Academy of Agricultural Sciences
The National Natural Science Foundation of China, grant number NSFC No. 31971879 and China Agriculture Research System grant number CARS-03
冰草(Agropyron cristatum L.,2n = 4x = 28,PPPP)携带的多样性外源基因是小麦遗传改良重要基因源。前期研究表明冰草1P染色体可以改良小麦株叶型,但在获得易位片段的基础上,外源片段导入不同背景小麦品种的叶型变化以及可能带来的不利遗传连锁累赘还不清楚。为进一步探究小麦-冰草T1PS.1AL和T1AS.1PL易位系在不同小麦背景中对主要农艺性状的遗传效应,本研究将小麦-冰草1PS、1PL易位系与百农607、川麦104、藁城8901等8个普通小麦品种进行杂交,对F2代分离群体进行农艺性状考察和分析。结果表明,冰草1PS染色体导入小麦后,降低了小麦的倒三叶长,株高也显著降低3~7cm,产量性状基本不受影响。冰草1PL染色体使小麦株高、旗叶、倒二叶和倒三叶显著降低,改变株型的同时,发现小麦的产量性状同时降低,具有连锁累赘。在冰草1P易位系中,后代的表型除与染色体本身所产生的效应有关外,也与小麦受体的遗传背景相关。我们发现在不同背景的小麦亲本杂交组合后代中,可以选出株叶型改良且产量性状不受影响的单株可用于小麦的遗传改良。总之,本研究报道了2个小麦-冰草1P易位系创新种质在株型改良上具有显著的遗传效应,为冰草1P染色体易位片段的利用提供指导。
Agropyron cristatum L. (2n = 4x = 28, PPPP) in a large collection of foreign excellent genes are valuable genetic resources for the genetic improvement of wheat. According to previous studies, the 1P chromosome of A.cristatum can improve plant leaf shape. However, it is unclear that the leaf shape changes and possible detrimental cumbersome effects from introduction of exotic segment in the existing of translocation fragment. This study is to detect the genetic diversity of wheat-A.cristatum translocation lines T1PS.1AL and T1AS.1PL on major agronomic characters in different wheat backgrounds, in which wheat-A.cristatum translocation lines 1PS and 1PL were crossed with eight common wheat varieties, followed by investigation and analysis of agronomic traits in F2 segregation population. The results showed that A.cristatum chromosome arm 1PS reduced the length of top three leaves. Also the plant height reduction level was 3-7cm while no significant difference on yield characters were discovered. Furthermore, the plant phenotype of the translocation line T1AS.1PL were shaped with a decrease in the grain yield than those of non-translocation plants. Offspring performance varies regardless of wheat backgrounds or different traits, which means that in the 1P translocation lines, the phenotype of offspring is related to the effect of chromosome itself and the genetic background of wheat receptor. We found that in the progeny of different wheat parental cross combinations, single plants with favourable leaf shape and unaffected yield traits could be selected for wheat genetic improvement. In conclusion, this study reported two innovative germplasm of wheat-A. cristatum 1P translocation lines had significant genetic effects on plant type improvement, which could guide the subsequent utilization of A. cristatum 1P chromosome translocation fragments.