Isopentenyl-transferases (IPT) are key enzymes that catalyze the biosynthesis of cytokinin and are also important rate-limiting enzymes of cytokinin. In plants, IPT exists in the form of ATP/ADP IPTs and TrnA-IPTS, and IPTs mediate the synthesis of two types of CKs in plants: the ATP/ADP pathway and the tRNA pathway. Ginger is an important vegetable that can been used as medicine and food, but it is tend to be affected by various biotic and abiotic stresses during its cultivation, which is detrimental to the safe production of ginger. Here, 10 ZoIPT were identified from ginger genome through systematic bioinformatics analysis, and they were named as ZoIPT1-ZoIPT10. Gene structure analysis showed that all ZoIPTs gene family members contain introns. The amino acid length of the encoded protein ranged from 283 (ZoIPT8) to 491(ZoIPT10) aa, the molecular weight was 31.14 (ZoIPT8) to 54.02 (ZoIPT10) kD, and the isoelectric point was 4.97 (ZoIPT8) to 9.37(ZoIPT6) pI. The analysis of protein characteristics showed that all ZoIPTs were hydrophilic. Subcellular localization analysis showed that 8 ZoIPTs were localized in chloroplasts and 2 ZoIPTs were localized in mitochondria and cytoplasm. Transcriptome data analysis showed that ZoIPTs had tissue specific expression patterns and could respond to stress such as disease and low temperature, ZoIPT3 and ZoIPT5 were highly expressed at different growth stages, different tissues, and in response to low temperature and disease stress. qRT-PCR analysis showed that ZoIPTs was responsive to drought, flooding and salt stress. Under flooding and salt stress, the expression of ZoIPT3 in rhizomes was significantly increased. Under drought stress, the expression of ZoIPT5 in leaves and rhizomes were significantly increased. In summary, through systematic identification, evolutionary analysis, characteristic analysis, promoter analysis, expression pattern analysis, and expression patterns analysis under drought, salt, and flooding stress, this study provided a theoretical basis for in-depth research on the biological functions of ZoIPT in regulating growth and development and stress resistance of ginger.