1.College of Agronomy,Henan Agricultural University/National Key Laboratory of Wheat and Maize Crop Science;2.Nanyang Academy of Sciences,Nanyang;3.China
National Key Research and Development Program (2019YFE0118300); Henan Major Science and Technology Projects(222102520001); China Postdoctoral Science Foundation (2022M711066)
小麦茎基腐病的发生给粮食生产造成巨大威胁,同时还会产生DON、NIV等毒素和次级代谢物严重影响食品安全和人畜健康,因此,筛选优良的抗病资源并挖掘抗性位点对小麦茎基腐病抗性育种具有重要意义。本研究采用小米培养基接种法对163份黄淮麦区小麦种质资源进行室内和田间茎基腐病抗性鉴定。研究结果表明,没有鉴定出高抗和免疫的小麦材料,但是不同材料的抗性能被明显区分,群体抗性整体上呈现正态分布趋势,病情指数分布在10.27~83.33之间；室内和田间鉴定结果较稳定,两环境病情指数的相关系数为0.79,说明室内鉴定结果可很好反应田间抗性情况；全基因组关联分析(GWAS)显示,显著SNP广泛分布于小麦各条染色体上,其中2A上最多,集中在725~763 Mb区段内；进一步集群分离分析法(BSA)结果显示,显著SNP集中在2A上的730~750 Mb区段内；综合来看,小麦2A染色体上730~750 Mb区段内可能存在显著调控小麦茎基腐病的抗性基因。本研究能够为小麦茎基腐病抗性材料筛选及抗病位点挖掘提供重要的参考意义。
In recent years, wheat fusarium crown rot(FCR)has been seriously appeared in many wheat regions of China, and posing a great threat to grain production. In addition, many toxins and secondary metabolites such as DON and NIV were produced during the disease occurrence, which brought serious problems to food safety and human or animal health. As the effort of current research mainly focuses on the isolation of FCR pathogens and the investigation of materials resistance, the progress of FCR study is far behind the speed of disease development. Therefore, it is of great significance to screen excellent resistant resources to control this disease. This study used millet grains as pathogen medium to inoculate the 163 wheat germplasm accessions in the Huanghuai valley of China. The results showed that there were no immune or highly resistant materials have been identified, but the resistance level estimated by disease index (DI) of the identified accessions was obvious difference with a normal distribution from 10.27 to 83.33. The correlation coefficient between seedling and field investigation results was 0.79 which indicated a highly correlation of seedling resistance to adult resistance. Furthermore, the genome-wide association study (GWAS) results showed that the significant SNPs were widely distributed on all the wheat chromosomes, and mainly focus on 2A with an interval from 725 Mb to 763Mb. The bulked segregant analysis (BSA) results suggested that the significant SNPs were mainly distributed from 730 Mb to 750Mb on 2A. Overall, the GWAS and BSA analysis indicated that a significant resistance locus on chromosome 2A from 730 Mb to 750Mb was identified to associate with FCR infection. This study can provide important reference for resistant materials investigation and resistance loci excavation on wheat FCR.