College of Agriculture,Shenyang Agricultural University,Shenyang
Shenyang Science and Technology Program for Seed Industry Innovation Special Project (21-110-3-16; 21-110-3-09-03; 22-318-2-01-02)；Breeding of New Early-Maturing and Machine-Harvestable Corn Varieties in the Central Liaoning Ecological Zone (2022JH1/10200001)
玉米穗长影响穗粒数，进而影响产量，是育种改良的重要目标性状之一。发掘与穗长性状相关QTL和基因对穗长性状进行遗传改良具有重要意义。本研究以一对穗长具有显著差异的高世代姊妹系为亲本，构建F2群体并自交得到F2:3，利用Maize6H-60K基因芯片分别对亲本及F2群体进行基因型分析，并通过ICIM、GCIM和dQTG -seq三种方法对F2和F2:3群体的穗长进行QTL定位。结果表明，三种方法共定位到7个与穗长相关QTL位点和43个QTNs，解释了2.04-10.24%的表型变异。交叉分析不同方法得出的定位结果，在6号染色体上发现一个调控穗长的稳定QTL qEL6.01。根据定位区间内的基因注释的结果，并结合参考基因的表达谱数据，共筛选出5个在雌穗中表达的候选基因Zm00001d035514、Zm00001d035526、Zm00001d035537、Zm00001d035553和Zm00001d035535，本研究结果为玉米穗长基因的克隆及育种改良奠定了基础。
he ear length of maize affects the number of grains per ear, and then affects the yield, which is one of the important target traits of breeding improvement. It is of great significance to explore QTL and genes related to ear length traits for genetic improvement of ear length traits. In this study, a pair of high-generation sister lines with significant differences in ear length were used as parents, the F2 population was constructed and self-crossed to obtain F2:3, and the parents and F2 population were genotyped using the Maize6H-60K Gene Chip, respectively, and the ear length of the F2 and F2:3 populations were identified as QTLs by the three methods of ICIM, GCIM and dQTG -seq. The results showed that 7 QTLs and 43 QTNs related to ear length were located by the three methods, which explained the phenotypic variation of 2.04-10.24%. A stable QTL qEL6.01 was found on chromosome 6 by cross- analysis of the mapping results obtained by different methods. According to the results of gene annotation in the qEL6.01 and the expression profile data of reference genes, five candidate genes Zm00001d035514, Zm00001d035526, Zm00001d035537, Zm00001d035553 and Zm00001d035535 were screened out. The results laid a foundation for the cloning and breeding improvement of maize ear length gene.