he ear length of maize affects the number of grains per ear, and then affects the yield, which is one of the important target traits of breeding improvement. It is of great significance to explore QTL and genes related to ear length traits for genetic improvement of ear length traits. In this study, a pair of high-generation sister lines with significant differences in ear length were used as parents, the F2 population was constructed and self-crossed to obtain F2:3, and the parents and F2 population were genotyped using the Maize6H-60K Gene Chip, respectively, and the ear length of the F2 and F2:3 populations were identified as QTLs by the three methods of ICIM, GCIM and dQTG -seq. The results showed that 7 QTLs and 43 QTNs related to ear length were located by the three methods, which explained the phenotypic variation of 2.04-10.24%. A stable QTL qEL6.01 was found on chromosome 6 by cross- analysis of the mapping results obtained by different methods. According to the results of gene annotation in the qEL6.01 and the expression profile data of reference genes, five candidate genes Zm00001d035514, Zm00001d035526, Zm00001d035537, Zm00001d035553 and Zm00001d035535 were screened out. The results laid a foundation for the cloning and breeding improvement of maize ear length gene.