To characterize the resistance of the novel rice mutant ALS179 to acetolactate synthase(ALS) inhibiting herbicides. In this study, wild-type Huahang31 (HH31), imidazolinone-tolerant rice ALS627 mutant and Ethyl Methyl Sulfone (EMS)-mutagenized novel rice mutant ALS179 were used as experimental materials to further determine the resistance of ALS179 to four types of ALS-inhibiting herbicides by seed coating and seedling spray treatment at different concentrations. The phenotypes and related enzyme activity indexes were further determined to investigate the resistance of the mutant ALS179. The results showed that the mutant ALS179 showed different degrees of resistance to benensulfuron, imazethapyr, bispyribac-sodium and pyroxsulam after seed coating and seedling spraying, and the activities of acetolactate synthase, peroxidase, catalase and superoxide dismutase tended to decrease with increasing herbicide concentration. The enzyme activities of ALS179 were higher than those of wild-type HH31 under all treatment conditions except for imazethapyr treatment at high concentrations.In this study, we found that the Ala179Val mutation conferred broad-spectrum resistance to ALS-inhibiting herbicides and provided genetic germplasm resources for the subsequent breeding of ALS-inhibiting herbicide broad-spectrum resistant rice lines.