Protein Disulfide Isomerase (PDI), a member of the thioredoxin family, is an oxidoreductase widely exists in animals, plants and microorganisms. In plants, the formation and isomerization of protein disulfide bonds are mainly catalyzed by disulfide bond proteins, which are crucial in participating in the correct assembly and folding of proteins. In this study, based on the gene sequence information of Arabidopsis PDI family genes, 21 PDI genes were identified from maize by homologous sequences alignment method, which were unevenly distributed on the 9 chromosomes of maize. The phylogenetic tree analysis showed that the maize PDI gene family was divided into four evolutionary branches and 11 developmental groups, and the gene structure and conserved motifs of the same development group were similar. The analysis of cis-acting elements showed that the promoters of the PDI gene family contained cis-acting elements that respond to stress, plant hormones and endosperm specific expression. The expression pattern analysis showed that the PDI gene family had a high expression level in embryo, endosperm and grain, and then showed a downward trend after reaching the peak in endosperm 10 and 15 days after pollination, and then showed an upward trend. Subcellular localization found that ZmPDIL1-1 was located in the endoplasmic reticulum. This study provides a reference for the functional analysis of PDI family genes in maize.