1.Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province,Harbin Normal University;2.Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/ National Engineering Laboratory for Crop Molecular Breeding,Beijing
蛋白质二硫键异构酶(PDI ,Protein Disulfide Isomerase)作为硫氧还蛋白家族的一员,是一种氧化还原酶,广泛存在于动物、植物及微生物中。在植物中,蛋白质二硫键的形成和异构主要由二硫键蛋白催化完成,在参与蛋白质正确组装折叠方面至关重要。本研究以拟南芥PDI家族基因序列信息为基础,利用同源序列比对方法,从玉米中鉴定出21个PDI基因,除8号染色体外,非均匀地分布在其余玉米9条染色体上。进化树分析表明,玉米PDI基因家族分为4个进化分支11个发育组,同一发育组的基因结构和保守基序相似。顺式作用元件分析表明,PDI基因家族启动子含响应逆境胁迫、植物激素及胚乳特异表达的顺式作用元件。表达模式分析表明,PDI基因家族在胚、胚乳和籽粒中表达量较高,在授粉后10天和15天的胚乳中达到高峰之后呈下降趋势,然后表达量又呈现上升趋势。亚细胞定位发现ZmPDIL1-1定位在内质网。本研究结果为玉米PDI家族基因功能研究提供参考。
Protein Disulfide Isomerase (PDI), a member of the thioredoxin family, is an oxidoreductase widely exists in animals, plants and microorganisms. In plants, the formation and isomerization of protein disulfide bonds are mainly catalyzed by disulfide bond proteins, which are crucial in participating in the correct assembly and folding of proteins. In this study, based on the gene sequence information of Arabidopsis PDI family genes, 21 PDI genes were identified from maize by homologous sequences alignment method, which were unevenly distributed on the 9 chromosomes of maize. The phylogenetic tree analysis showed that the maize PDI gene family was divided into four evolutionary branches and 11 developmental groups, and the gene structure and conserved motifs of the same development group were similar. The analysis of cis-acting elements showed that the promoters of the PDI gene family contained cis-acting elements that respond to stress, plant hormones and endosperm specific expression. The expression pattern analysis showed that the PDI gene family had a high expression level in embryo, endosperm and grain, and then showed a downward trend after reaching the peak in endosperm 10 and 15 days after pollination, and then showed an upward trend. Subcellular localization found that ZmPDIL1-1 was located in the endoplasmic reticulum. This study provides a reference for the functional analysis of PDI family genes in maize.