1.College of Horticulture,Nanjing Agricultural University,Nanjing;2.Institute of Vegetable Crops,Jiangsu Academy of Agricultural Sciences,Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanjing
The Natural Science Foundation of Jiangsu Province(BK20221429)，Key Research and Development Program of Jiangsu Province(BE2021377), the Independent Innovation Foundation of Agri-cultural Sciencesof Jiangsu Province（ CX(22)3160）
Whirly是一类能与单链 DNA 分子结合的植物特异转录因子，在细胞核以及细胞器内都起着广泛且复杂的作用。为探究茄子Whirly基因的功能和进化关系，本研究开展了基因家族成员生物信息学鉴定，包括系统进化关系、基因结构、保守基序及启动子中的顺式作用元件，分析了其在不同组织、外源激素处理和逆境胁迫下表达模式。结果表明，茄子Whirly基因家族包含2个家族成员，分别命名为SmWHY1和SmWHY2，它们与番茄中Whirly基因亲缘关系最近。SmWHY1和SmWHY2在不同组织中均能表达，在叶片中SmWHY1的表达水平高于SmWHY2，在其它组织中SmWHY2的表达水平高于SmWHY1。2个基因的表达均受到脱落酸、茉莉酸甲酯、水杨酸、低温胁迫和病原菌诱导，但不同处理或基因型响应程度存在差异。以上结果表明SmWHY1和SmWHY2可能在调控茄子生长发育和逆境响应中具有重要作用。
Whirly is a kind of plant specific transcription factor which can combine with single strand DNA molecules, and plays a wide and complex role in the nucleus and organelles.To understand the function and evolutionary relationship of eggplant Whirly genes, bioinformatic analysis were performed to identify and characterize Whirly genes in eggplant genome, including the evolutionary relationship, gene structure, conservative motifs, cis acting elements in promoters. The expression mode of the genes wasalso analyzed in different tissues and under the treatments of exogenous hormone, low temperature and pathogen. The results showed that there were two Whirly gene members in eggplant genome, named SmWHY1 and SmWHY2, which were closely related to the tomato Whirly genes. Their expression can be detected in various tissues. SmWHY1 showed higher expression level than SmWHY2 in leaves, while SmWHY2 showed higher expression level than SmWHY1 in other tissues. The expression of two genes could be induced by abscisic acid, methyl jasmonate, salicylic acid, low temperture and pathogen, and the responses varied by gene and/or by treatments .Above results indicated that SmWHY1 and SmWHY2 may played important roles in the regulation of growth, development, and response to stress.