高粱株高相关基因SbPH11分子标记的开发和应用
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作者单位:

1.山西农业大学高粱研究所;2.中国科学院植物研究所;3.青海牛必乐农牧科技有限公司;4.青海省饲草料技术推广站

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西宁市重点研发与转化计划(2022-Y-18);中国科学院战略性先导科技专项(XDA26030201);财政部和农业农村部:国家现代农业产业技术体系(CARS-06)


Development and Application of Molecular Markers of Sorghum Plant Height Related Gene SbPH11
Author:
Affiliation:

1.Sorghum Research Institute, Shanxi Agricultural University;2.Institute of Botany,Chinese Academy of Sciences;3.Qinghai Cattle Pierer Husbandry Technology CoLtd;4.Qinghai Feed and Forage Technology Promotion Station

Fund Project:

The Key Research and Development Program of Xining City(2022-Y-18);The Strategic Priority Research Program of the Chinese Academy of Sciences(XDA26030201); China Agriculture Research System of MOF and MARA(CARS-06)

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    摘要:

    高粱具有较强的耐盐碱特性,是最具耐盐碱品种开发潜力的饲用作物之一。生物量是饲用高粱的重要性状,株高与生物量呈正相关性。本研究以237份高粱自交系关联群体为材料,基于株高关联基因SbPH11的两个功能性SNP位点组合的单倍型开发了KASP分子标记,利用该标记对部分供试自交系关联群体材料和30份高粱种质资源进行了基因分型和表型验证,结果证实开发的KASP分子标记可以准确地鉴定出SbPH11两个功能性SNP位点的基因型。两个SNP位点组合的单倍型共3种:SbPH11-Hap1、SbPH11-Hap2和SbPH11-Hap3,SbPH11-Hap2 所对应的高粱材料的株高显著高于SbPH11-Hap1和SbPH11-Hap3所对应的高粱材料的株高,SbPH11-Hap1的高粱材料株高显著高于SbPH11-Hap3的高粱材料株高。该KASP分子标记可高效准确地预测不同高粱种质资源的株高类型,可应用于高粱株高的早期筛选和分子标记辅助选择育种。

    Abstract:

    Sorghum is a type of forage crops with the most potential of saline-tolerance, which is known for its strong tolerance to high level of salt and alkaline. Biomass is an important trait of forage sorghum and plant height is positively correlated with it. In this study, a KASP marker associated to plant height was developed based on the haplotype of two functional SNP loci of gene SbPH11 using an association mapping population including of 237 sorghum inbred lines. The marker was then used to genotype a subset of the association mapping population and other 30 sorghum germplasms resources, and the results confirmed that the KASP marker could accurately identify the two functional SNP loci of SbPH11. There are three haplotypes in SbPH11: SbPH11-Hap1, SbPH11-Hap2 and SbPH11-Hap3, the plant height of sorghum corresponding to SbPH11-Hap2 was significantly higher than that of SbPH11-Hap1 and SbPH11-Hap3, and the plant height of sorghum corresponding to SbPH11-Hap1 was significantly higher than that of SbPH11-Hap3. These results suggested that the KASP marker can efficiently and accurately predict the plant height of different sorghum germplasm resources, and can be applied to early screening and molecular marker-assisted selection in the breeding for sorghum plant height.

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  • 收稿日期:2023-06-11
  • 最后修改日期:2023-07-24
  • 录用日期:2023-07-31
  • 在线发布日期: 2023-08-04
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