College of Agronomy, Henan Agricultural University/Maize Industry Integration Engineering Technology Research Center of Henan Province
Major public welfare projects in Henan Province（201300111100）；Natural Science Foundation project of Henan Province（212300410356）；Technical system of maize industry in Henan Province（HARS-22-02-S）；Henan Province agricultural improved seed joint research project （2022010201）
高油玉米胚较大，具有较高的营养价值。本研究对高油玉米自交系GY220授粉后15 d（G15）、25 d（G25）、35 d（G35）的胚蛋白质组进行双向凝胶电泳分离，利用串联质谱对分离蛋白质进行鉴定，并分析了差异表达蛋白功能。结果表明在授粉后三个时期鉴定到41个差异蛋白，其中G25/G15上调18个，下调19个；G35/25上调18个，下调25个；G35/G15上调7个，下调22个。通过GO注释分析和KEGG富集分析显示差异表达蛋白主要在小分子代谢、氧化还原和碳水化合物代谢等过程富集。根据玉米自交系B73转录组数据库，结合自交系GY220中差异蛋白编码基因RT-PCR表达分析，表明在胚发育后期差异蛋白甘油-3-磷酸脱氢酶、果糖激酶表达量增加，并与大豆、花生、油菜中的相关基因高度同源。本研究为进一步提升玉米籽粒品质和挖掘胚发育功能基因奠定基础。
Abstract: The embryo in high-oil maize is larger and has higher nutritional value. In this study, the embryonic proteome at 15 (G15), 25 (G25) and 35 (G35) days after pollination was isolated by two-dimensional electrophoresis in high-oil maize inbred line GY220, and the isolated proteins were identified by tandem mass spectrometry. The function of differentially expressed proteins were analyzed. The results showed that 41 differential proteins were identified in the three stages after pollination, among which 18 proteins were up-regulated expression and 19 were down-regulated expression by comparing G25 and G15, and 18 proteins upregulation and 25 down-regulation in G35/25, 7 proteins up-regulation and 22 down-regulation in G35/15. GO annotation analysis and KEGG enrichment analysis showed that the differential proteins were mainly enriched in small molecule metabolism, REDOX and carbohydrate metabolism. According to the transcriptomic database of inbred line B73 and RT-PCR analysis of differential protein encoding gene in inbred line GY220 showed that the expression of differential protein, glycerin-3-phosphate dehydrogenase and fructokinase, significantly increased in late embryo development, and they were highly homologous with related genes in soybean, peanut and rape. This study laid a foundation for further improving grain quality and mining functional genes of embryo development in maize.