1.School of Life Sciences, Guangzhou University/Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design;2.Beijing Zhongnong Futong Horticulture Co,Ltd
National Natural Science Foundation of China（32272073）
土地盐渍化对大豆的产量和品质产生了极大的负面影响，培育耐盐大豆品种是改善和提高盐胁迫下大豆产量和品质的有效途径之一。ERF转录因子对植物响应逆境胁迫十分重要，但在大豆中相关研究报道较少。基于已报道的盐胁迫处理下的RNA-seq数据、549份大豆重测序数据及耐盐指数数据，以及Soybean Expression Atlas数据库中大豆组织表达数据，在大豆中鉴定能够响应盐胁迫的ERF基因。同时，在549份大豆重测序数据中鉴定响应盐胁迫ERF基因的优异等位变异，并分析其驯化与人工选择规律。对大豆植株盐胁迫下转录组数据分析，发现153个ERF基因中有40个受到盐胁迫后差异表达，其中11个基因上调表达，29个基因下调表达；11个上调表达的ERF基因中，有8个基因在根中富集表达，并分布在6条染色体上。对8个在根中表达的ERF基因在549份自然群体中鉴定自然等位变异，发现只有3个ERF基因产生了自然变异，其中ERF158H1，ERF166H2，ERF170H1单倍型是优异的等位变异，能够显著促进大豆对盐的耐性。对优异等位变异的核苷酸多态性分析表明，ERF170H1在大豆驯化的过程中受到了微弱的人工选择，而ERF158H1和ERF166H2在驯化的过程中发生了逐渐减少或丢失的现象。因此，我们的研究鉴定了大豆中响应盐胁迫的ERF基因及其优异等位变异，对丰富和完善大豆响应盐胁迫的分子机制具有重要意义，为培育耐盐大豆品种提供重要的基因资源和育种方案。
Soil salinization has a significant negative impact on the yield and quality of soybean. It is one of the effective ways to improve the yield and quality of soybeans under salt stress by breeding salt-tolerant soybean varieties. ERF transcription factors play an important role in plant response to biotic and abiotic stresses, however, there are few relevant studies in soybean. Based on RNA-Seq data under salt stress, 549 Soybean resequencing data and salt tolerance index data that have been reported in our previous study, as well as the soybean tissue expression data in the Soybean Expression Atlas database, the ERF genes that can respond to salt stress in soybean were identified. Meanwhile, the excellent alleles of ERF genes that can respond to salt stress were identified in 549 soybean resequencing data, and the domestication and artificial selection have also been analyzed. U. The transcriptome data of soybean plants under salt stress showed that 40 of 153 ERF genes were differentially expressed after salt stress, among which 11 genes were up-regulated and 29 genes were down-regulated. Among the 11 up-regulated ERF genes, 8 genes were enriched in roots and distributed on 6 chromosomes. The natural allelic variations of 8 ERF genes that mainly expressed in roots, were identified in 549 natural soybean populations. The results showed that only three ERF genes exist natural variations, among which ERF158H1, ERF166H2, ERF170H1 haplotypes were excellent allelic variations, which could significantly promote salt tolerance of soybean. Nucleotide polymorphism analysis of those excellent alleles showed that ERF170H1 was weakly artificially selected during soybean domestication, while ERF158H1 and ERF166H2 were gradually reduced or lost during domestication process. Therefore, Eight ERF genes that may be involved in the response of soybean to salt stress were identified, and three of them were found to have excellent alleles that can significantly promote soybean salt tolerance. Our findings provide a great significance to enrich and improve the molecular mechanism of soybean response to salt stress and breeding programs for salt-tolerant soybean varieties.