Bulked Segregant Analysisis (BSA) is a method of identifying gene loci through constructing the DNA pools with extreme traits. Because of its "fast, accurate, economical and practical" characteristics, BSA has become a widely used method of gene mapping. With the rise of high-throughput sequencing technology, BSA based on whole-genome resequencing has been widely applied in locating many genes for agronomic traits of grain and oil crops, vegetables, flowers and other species. This study briefly introduced the methods and procedures of BSA, summarized the research progress in genetic mapping of agronomic traits, stress resistance traits and male sterility traits in soybean, and discussed the opportunities and challenges of BSA under the background of NGS, as well as the development trend of BSA in soybean molecular marker assisted selection (MAS) breeding. This study will provide theoretical basis for the cultivation of high quality soybean varieties.