玉米愈伤组织形成的转录组分析
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1.甘肃农业大学农学院/省部共建干旱生境作物学国家重点实验室/甘肃省作物遗传改良与种质创新重点实验室;2.中国农业科学院作物科学研究所

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中国农业科学院创新工程


Transcriptome Analysis of Maize Embryonic Callus Formation
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Affiliation:

1.College of Agronomy,Gansu Agricultural University/Gansu Provincial Key Laboratory of Aridland Crop Science/Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement;2.Institute of Crop Sciences,Chinese Academy of Agricultural Sciences

Fund Project:

the Agricultural Science and Technology Innovation Program of CAAS

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    摘要:

    玉米遗传转化受体主要是未成熟幼胚,经过组织培养形成胚性愈伤组织,进而分化成苗。目前,对玉米胚性愈伤组织形成的分子机理的了解还不深入。为了挖掘调控玉米胚性愈伤组织形成的基因,本研究选取玉米自交系CAL28×郑58的F3群体中不同表型的愈伤组织进行RNA-seq分析。与愈伤发生差的II型愈伤相比,愈伤发生好的I型愈伤中共检测到4419个差异表达基因,其中1571个基因上调,2848个基因下调。GO富集分析结果表明,差异表达基因主要富集在应激反应、细胞组分和催化活性等通路中。KEGG富集分析表明,差异表达基因主要富集在苯丙烷生物合成途径和植物激素信号转导途径。植物激素信号转导途径中SAUR家族、ZIM家族结构域蛋白、IAA生长素响应因子在I型愈伤中上调表达。DEGs中共有隶属56个转录因子家族的2968个转录因子,其中与愈伤形成相关的AP2、WOX和LBD转录因子家族基因中的EREB24、EREB53、EREB184、EREB206、ZmLBD10、ZmLBD24、ZmLBD31、ZmLBD32等在Ⅰ型愈伤中的表达量显著上调,对这些靶标基因进行遗传操作有可能促进玉米愈伤组织形成。本研究发现的靶标基因具有较大的应用潜力,研究结果也为解析玉米愈伤组织形成的分子机理提供理论参考。

    Abstract:

    The immature embryos of maize can form embryogenic callus through tissue culture, and are usually used as explants for genetic transformation. It is not well known about the molecular mechanism of maize embryonic callus formation. To reveal the potential genes involved in the callus formation of maize, type I calli with well growth and type II calli with poor growth from F3 embryos of maize inbred line CAL28× Zheng58 were selected for RNA-seq analysis. Compared to type II calli, there were 4419 differentially expressed genes (DEGs) in type I calli, including 1571 upregulated genes and 2848 downregulated genes. The results of GO enrichment analysis showed that DEGs were mainly enriched in response to stimulus, cell components and catalytic activity pathways. KEGG enrichment analysis showed that the enrichment of DEGs were mainly involved in phenylpropanoid biosynthesis and the plant hormone signal transduction pathway. The SAUR family, ZIM family and IAA growth hormone response factors were upregulated in type I callus. A total of 2968 transcription factors belonging to 56 transcription factor families in DEGs were significantly enriched. The expression levels of EREB24, EREB53, EREB184, EREB206, ZmLBD10, ZmLBD24, ZmLBD31 and ZmLBD32, which are from AP2, WOX and LBD transcription factor families, were significantly up-regulated in type Ⅰ callus. Genetic manipulation of these target genes may promote the formation of maize callus.The results provide theoretical reference for the analysis of molecular mechanism of maize callus formation, and the identified candidate DEGs would have great application potential.

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历史
  • 收稿日期:2022-12-17
  • 最后修改日期:2023-02-22
  • 录用日期:2023-03-14
  • 在线发布日期: 2023-03-31
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