255份马铃薯种质晚疫病广谱Rpi基因标记检测及抗性田间验证
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云南农业大学薯类研究所

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云南省科技计划项目重大科技专项(202102AE090018);国家产业技术体系(CARS-09-15P)


Detection of Late Blight Broad-spectrum Rpi Gene Markers and Field Verification of Resistance in 255 Potato Germplasm
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Tuber and Root Crop Research Institute,Yunnan Agricultural University

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Major science and technology project of Yunnan Province(202102AE090018);National industrial technology system of China(CARS-09-15P)

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    摘要:

    马铃薯(Solanum tuberosum L.)是云南省的第三大粮食作物,晚疫病严重威胁马铃薯的生产,防治晚疫病最好的策略是种植抗性品种。本研究对255份马铃薯种质检测已报道的6个广谱Rpi基因分子标记,并鉴定田间晚疫病抗性。结果表明,含R8、RB、Rpi-blb2、Rpi-sto1、Rpi-sto1、Rpi-vnt1.1分子标记的材料分别有69、53、51、12、23、76份;81份材料聚合了多个广谱Rpi基因分子标记,其中77份来源于国际马铃薯中心(CIP)。田间抗性鉴定结果表明:255份材料中,高抗、中抗、中感和高感材料分别有68、35、68、84份,高抗与中抗材料分别有60份和33份源于CIP。国内种质与CIP种质材料相比,抗病与聚合多个广谱Rpi基因分子标记的种质缺乏。结果还表明,R8分子标记在晚疫病田间抗性中起主要贡献,与田间抗性符合程度较高,并在聚合其它广谱Rpi基因分子标记时,符合程度与抗性会随之提高。本研究检测了255份材料的6个广谱Rpi基因分子标记组成,筛选出一批抗性材料,证明了R8分子标记具有较高的晚疫病田间抗性符合程度,可用于分子标记辅助选择,为抗病育种与分子标记辅助选择育种提供了科学依据。

    Abstract:

    Potato(Solanum tuberosum L.) is the third most important food crop in Yunnan Province. Late blight has always been a devastating disease in potato production. Use of resistant varieties is the most fundamental and effective way to control the disease. In this study, screening of broad-spectrum Rpi genes molecular marker and evaluation of late blight resistance upon field in 255 potato genotypes. The results showed that there were 69, 53, 51, 12, 23 and 76 varieties(lines) containing R8, RB, Rpi-blb2, Rpi-sto1, Rpi-sto1 and Rpi-vnt1.1, respectively. Eighty-one of the varieties(lines) were polymerized with multiple broad-spectrum Rpi gene markers, and 77 were derived from CIP. The results of field resistance identification showed that: Among the 255 varieties (lines), 68, 35, 68 and 84 varieties(lines) were of high resistance, medium resistance, medium sensitivity and high sensitivity, respectively. Thirty-three moderately resistance and 60 highly resistance varieties(lines) were derived from CIP, respectively. Compared with CIP, the domestic germplasm is lack of resistance and polymerization of multiple broad spectrum Rpi gene markers. R8 marker played a major role in field resistance to late blight, which was highly consistent with field resistance. Potato polymerized R8 markers and other broad-spectrum Rpi gene markers can improve the degree of resistance compliance and field resistance. This study demonstrated that R8 marker have a high degree of compatibility with field resistance to late blight and could be used for marker assisted selection, which could provide scientific basis for the future popularization and application of new varieties(lines) and resistance breeding in Yunnan Province.

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  • 收稿日期:2022-12-04
  • 最后修改日期:2023-01-10
  • 录用日期:2023-01-17
  • 在线发布日期: 2023-02-03
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