Abstract:The crops, including apple, pear, citrus, banana and other fruit tree crops, and potato, sweet potato, cassava and other tuber crops, as well as rubber, coconut, oil palm and other tropical economic crops, have to be planted through propagules such as plants, roots, tubers, bulbs, and seed stems. Based on the difference on long-term preservation strategy, these species are referred "field genebank preservation crops". Benefits from the nearly 40-years development, a technical strategy on safe preservation of these germplasm resources in National Field Genebanks of China has been estimated, including the facilities, technical procedures on sampling and preservation, as well as backup preservation. With the application of newly-established in vitro and cryopreservation facilities in National Crop Genebank of China, a preliminary integrated preservation system, including the National Field Genebanks as the core, in vitro genebank, cryopreservation genebank and in situ protection sites, is able to guarantee the long-term safe preservation and continuous utilization of diversified field genebank preservation crops. By December of 2021, forty-three National Field Genebanks have maintained 69,504 accessions of 1,469 species or subspecies, hosting the largest number of accessions in the world. This article reviews the reproductive characteristics of the conserved germplasm resources in National Field Genebanks of China, the factors that affect safe preservation and the corresponding strategies that address to these factors, as well as the practical applications and prospects of the preservation facilities system, in order to provide insights on safe preservation of these special germplasm resources in China.