水稻DUF761基因家族成员鉴定与表达谱分析
作者:
作者单位:

湖南农业大学农学院/水稻油菜抗病育种湖南省重点实验室/作物基因工程湖南省重点实验室

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金项目(31401699);湖南省自然科学基金项目(2021JJ30314);湖南创新型省份建设专项经费,岳麓山种业创新项目(2021NK1012)


Identification and Expression Profile Analysis of DUF761 Gene Family Members in Rice
Author:
Affiliation:

College of Agronomy, Hunan Agricultural University/Hunan Provincial Key Laboratory of Rice and Rapseed Breeding for Disease Resistance/Crop Gene Engineering Key Laboratory of Hunan Province

Fund Project:

The National Natural Science Foundation of China (31401699), The Natural Science Foundation of Hunan Province, China (2021JJ30314),Hunan Innovative Province Construction Special Fund Grant,Yuelu Mountain Seed Industry Innovation Project (2021NK1012)

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    DUF761家族是植物中一个未知功能结构域蛋白(Domains of unknown function,DUF)家族,其在水稻中的功能从未被探索。本研究通过生物信息学分析在水稻全基因组共鉴定到33个水稻DUF761基因家族成员。系统发育分析将这些成员分为三个亚家族。保守基序Motif-1分布于每一个亚家族的每一个成员中。在OsDUF761基因启动子区域共发现15种与胁迫响应、激素响应和发育相关的顺式作用元件。有30个基因的启动子区域含有ABRE元件(脱落酸响应),其中OsDUF761-21的启动子含有16个ABRE元件。组织表达谱检测结果表明,不同的OsDUF761基因成员在水稻中具有相似或者迥异的组织表达谱。在ABA、JA、低温、干旱和稻瘟菌分别处理水稻后,水稻中分别有12、13、24、24和18个OsDUF761基因的转录表达水平发生显著变化;其中,有8个基因在ABA处理和干旱处理后表现出共同的显著上调或者下调响应趋势;有6个基因在JA处理和稻瘟菌处理后表现出共同的响应趋势。本研究对水稻DUF761基因家族进行了全面的分析,这有助于进一步明确DUF761家族成员的生物学功能。

    Abstract:

    The DUF761 family members contain a domain of unknown function (DUF) protein in plants, and these functions in rice remain un-described. In this study, 33 family members in rice genome have been identified by bioinformatics pipeline. Phylogenetic analysis suggested three subfamilies, while Motif-1, a conserved motif was present in each member of each subfamily. Fifteen types of cis-acting elements related to stress response, hormone response and development were found in the promoters of these OsDUF761 genes. The promoters of 30 OsDUF761 genes contain the ABRE element (abscisic acid response), and the promoter of a member OsDUF761-21 contains 16 ABRE elements. Tissue expression profile analysis showed that OsDUF761 gene members represented similar or distinct tissue expression profile patterns in rice. The transcriptional expression levels of 12, 13, 24, 24 and 18 OsDUF761 genes in rice were significantly changed after ABA, JA, low temperature, drought and rice blast treatments, respectively. Eight genes showed a common significant up- or down-regulation response trend after ABA treatment and drought treatment. Six genes showed a common response trend after JA treatment and M. oryzae treatment. Collectively, by conducting a comprehensive analysis of the rice DUF761 gene family members, this study provided insights for future deciphering their biological functions in rice.

    参考文献
    相似文献
    引证文献
引用本文

杜 强,韩玲玲,高芙蓉,等.水稻DUF761基因家族成员鉴定与表达谱分析[J].植物遗传资源学报,2022,23(5):1487-1499.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2022-03-31
  • 最后修改日期:2022-04-29
  • 录用日期:2022-06-08
  • 在线发布日期: 2022-09-09
  • 出版日期:
您是第位访问者
ICP:京ICP备09069690号-23
京ICP备09069690号-23
植物遗传资源学报 ® 2024 版权所有
技术支持:北京勤云科技发展有限公司