花生种子大小相关性状QTL定位及与出仁率的关系
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中国农业科学院油料作物研究所/农业农村部油料作物生物学与遗传育种重点实验室

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国家自然科学基金项目(面上项目,重点项目,重大项目)


QTL mapping for seed size related traits and its relationship with shelling percentage in peanut
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Oil Crops Research Institute,China Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops,the Ministry of Agriculture and Rural Affairs

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The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)

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    摘要:

    花生是我国重要的油料作物之一,高产是花生育种的重要目标性状,种子大小和出仁率是花生产量构成的因素。定位种子大小性状相关QTL,并分析其与出仁率的关系,将为花生高产分子育种奠定基础。本研究以种子大小差异显著的徐花13和中花6号为亲本构建的重组自交系群体(RIL)为材料,连续3年对种子大小性状进行考察,种子长、种子宽和百仁重在RIL群体中变异广泛,并且性状间均极显著正相关。通过前期构建的遗传图谱,共检测到52个QTL位点,贡献率为3.09%~17.34%,其中qSLA05.2和qHSWA05.2, qSWA07.1 和qHSWA07为两个性状共定位的主效QTL并且能在多个环境下被重复检测到。qHSWA05.2和 qHSWA07的有利等位基因分别来自于母本和父本,利用连锁标记发现这两个有利等位基因组合在RIL群体中能使百仁重增加33.80±1.19 g。结合前期利用该群体出仁率QTL定位结果,发现出仁率与种子大小主效QTL除存在共定位外,也存在特有QTL。上述结果为精细定位和分子标记辅助育种以及多性状协同改良花生产量奠定了基础。

    Abstract:

    Peanut is one of the important oilseed crops in China. High yield is an important breeding objective. Seed size, and shelling percentage are the factors effecting of peanut yield. QTL mapping for seed size and analyzing their relationship with shelling percentage will lay a foundation for high yield molecularbreeding. In this study, the seed size of a recombinant inbred line (RIL) population ( Xuhua 13×Zhonghua 6) were were investigated for three consecutive years. The result showed that seed length (SL), seed width (SW), and hundred seed weight (HSW) varied widely in RIL population, and there were significant positive correlation among the three traits. Based on the genetic map, 52 QTL were detected with 3.09%-17.34% phenotypic variations explained (PVE), of which qSLA05.2 and qHSWA05.2, qSWA07.1 and qHSWA07 are co-localization major QTLs and can be repeatedly detected in multiple environments. Favorable alleles of qHSWA05.2 and qHSWA07 were derived from the female and male parents, respectively. A combination of the two favorable alleles using the linked markers was verified to increase hundred seed weight by 33.80±1.19g in the RIL population. Combined with the QTL mapping results of shelling percentage, it was found that there were co-localization and specific QTL among the traits. These results lay a foundation for fine mapping, molecular marker-assisted breeding and synergistic improvement of peanut yield.

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  • 收稿日期:2022-03-23
  • 最后修改日期:2022-04-19
  • 录用日期:2022-05-06
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